• Product nameAnti-Tissue Factor antibody
    See all Tissue Factor primary antibodies
  • Description
    Rabbit polyclonal to Tissue Factor
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 33 - 295 of Human Tissue Factor (BC011029).

  • Positive control
    • WB: Human fetal kidney, brain, skin and lung lysates IHC-P: Human fetal kidney tissue



Our Abpromise guarantee covers the use of ab104513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/500.


  • FunctionInitiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • Sequence similaritiesBelongs to the tissue factor family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD142 antibody
    • CD142 antigen antibody
    • Coagulation factor III (thromboplastin tissue factor) antibody
    • Coagulation factor III antibody
    • F3 antibody
    • FLJ17960 antibody
    • TF antibody
    • TF_HUMAN antibody
    • TFA antibody
    • Thromboplastin antibody
    • Tissue factor antibody
    see all

Anti-Tissue Factor antibody images

  • All lanes : Anti-Tissue Factor antibody (ab104513) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal skin lysate
    Lane 4 : Human fetal lung lysate

    Predicted band size : 33 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab104513, at a 1/100 dilution, staining Tissue Factor in formalin-fixed, paraffin-embedded Human fetal kidney tissue, showing membrane staining.

References for Anti-Tissue Factor antibody (ab104513)

This product has been referenced in:
  • Cassidy MR  et al. Histone deacetylase inhibitors decrease intra-abdominal adhesions with one intraoperative dose by reducing peritoneal fibrin deposition pathways. Surgery 155:234-44 (2014). WB ; Rat . Read more (PubMed: 24239397) »
  • Darbousset R  et al. Tissue factor-positive neutrophils bind to injured endothelial wall and initiate thrombus formation. Blood 120:2133-43 (2012). Mouse . Read more (PubMed: 22837532) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - other (aortic vascular smooth muscle cells)
Specification aortic vascular smooth muscle cells
Treatment Indoxy sulfate 0.25-100 ug/ml
Blocking step Milk as blocking agent for 45 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Dr. moshe shashar

Verified customer

Submitted Jul 18 2014

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

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Thanks for the additional information.

I have initiated the request for a replacement of one vial of ab104513. Please contact me again if you have any further questions.

Thank you for submitting your completed questionnaire.

It does appear that this antibody is not working as expected. If you have received this antibody within the past 6 months or so I would be happy to offer a replacement.

In your ...

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Application Flow Cytometry
Sample Rat Cell (Plasma Microparticles)
Specification Plasma Microparticles
Preparation Cell harvesting/tissue preparation method: Centrifuge whole blood 1,500xg 15 min, then centrifuge the supernatant again at 13,000xg 2 min, and retain supernatant
Sample buffer: Annexin Binding Buffer
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy MP samples without label, and sample with secondary antibody only

Abcam user community

Verified customer

Submitted Feb 23 2012

I am very pleased to hear you would like to accept our offer and test ab104513 in flow cytometry using rat samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forflow cyto...

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