Overview

  • Product name
    Anti-Tissue Factor antibody
    See all Tissue Factor primary antibodies
  • Description
    Rabbit polyclonal to Tissue Factor
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 33 - 295 of Human Tissue Factor (BC011029).

  • Positive control
    • WB: Human fetal kidney, brain, skin and lung lysates IHC-P: Human fetal kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab104513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/500.

Target

Anti-Tissue Factor antibody images

  • All lanes : Anti-Tissue Factor antibody (ab104513) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal skin lysate
    Lane 4 : Human fetal lung lysate


    Predicted band size : 33 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab104513, at a 1/100 dilution, staining Tissue Factor in formalin-fixed, paraffin-embedded Human fetal kidney tissue, showing membrane staining.

References for Anti-Tissue Factor antibody (ab104513)

This product has been referenced in:
  • Guiraut C  et al. Perinatal Arterial Ischemic Stroke Is Associated to Materno-Fetal Immune Activation and Intracranial Arteritis. Int J Mol Sci 17:N/A (2016). IHC-P ; Rat . Read more (PubMed: 27898024) »
  • Mizugishi K  et al. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy. J Biol Chem 290:2053-68 (2015). IHC-P ; Mouse . Read more (PubMed: 25505239) »

See all 4 Publications for this product

Product Wall

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - other (aortic vascular smooth muscle cells)
Specification
aortic vascular smooth muscle cells
Treatment
Indoxy sulfate 0.25-100 ug/ml
Blocking step
Milk as blocking agent for 45 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Dr. Moshe Shashar

Verified customer

Submitted Jul 18 2014

Thank you for contacting us.


I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
<...

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Thanks for the additional information.

I have initiated the request for a replacement of one vial of ab104513. Please contact me again if you have any further questions.

Thank you for submitting your completed questionnaire.

It does appear that this antibody is not working as expected. If you have received this antibody within the past 6 months or so I would be happy to offer a replacement.

In your ...

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Application
Flow Cytometry
Sample
Rat Cell (Plasma Microparticles)
Specification
Plasma Microparticles
Preparation
Cell harvesting/tissue preparation method: Centrifuge whole blood 1,500xg 15 min, then centrifuge the supernatant again at 13,000xg 2 min, and retain supernatant
Sample buffer: Annexin Binding Buffer
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
MP samples without label, and sample with secondary antibody only
Username

Abcam user community

Verified customer

Submitted Feb 23 2012

I am very pleased to hear you would like to accept our offer and test ab104513 in flow cytometry using rat samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forflow cyto...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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