Anti-Tissue Factor antibody (ab48647)

Overview

  • Product nameAnti-Tissue Factor antibody
    See all Tissue Factor primary antibodies
  • Description
    Rabbit polyclonal to Tissue Factor
  • Tested applicationsSuitable for: RIA, IP, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human, African Green Monkey
  • Immunogen

    Sf21 expressed human Tissue Factor.

Properties

Applications

Our Abpromise guarantee covers the use of ab48647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • FunctionInitiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • Sequence similaritiesBelongs to the tissue factor family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD142 antibody
    • CD142 antigen antibody
    • Coagulation factor III (thromboplastin tissue factor) antibody
    • Coagulation factor III antibody
    • F3 antibody
    • FLJ17960 antibody
    • TF antibody
    • TF_HUMAN antibody
    • TFA antibody
    • Thromboplastin antibody
    • Tissue factor antibody
    see all

Anti-Tissue Factor antibody images

  • ab48647 staining Tissue Factor in Cos-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/300) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-Tissue Factor antibody (ab48647)

ab48647 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Sample Monkey Cell (COS)
Specification COS
Permeabilization Yes - 0.1% Triton
Fixative Paraformaldehyde
Username

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Verified customer

Submitted Jan 03 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Monkey Cell (cos-7)
Specification cos-7
Permeabilization Yes - 0.1% Triton X100
Fixative Paraformaldehyde
Username

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Verified customer

Submitted Sep 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"