Anti-Tissue Factor antibody (ab48647)

Overview

  • Product name
    Anti-Tissue Factor antibody
    See all Tissue Factor primary antibodies
  • Description
    Rabbit polyclonal to Tissue Factor
  • Tested applications
    Suitable for: RIA, IP, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human, African green monkey
  • Immunogen

    Sf21 expressed human Tissue Factor.

Properties

Applications

Our Abpromise guarantee covers the use of ab48647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • Sequence similarities
    Belongs to the tissue factor family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD142 antibody
    • CD142 antigen antibody
    • Coagulation factor III (thromboplastin tissue factor) antibody
    • Coagulation factor III antibody
    • F3 antibody
    • FLJ17960 antibody
    • TF antibody
    • TF_HUMAN antibody
    • TFA antibody
    • Thromboplastin antibody
    • Tissue factor antibody
    see all

Anti-Tissue Factor antibody images

  • ab48647 staining Tissue Factor in Cos-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/300) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-Tissue Factor antibody (ab48647)

ab48647 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Sample
Monkey Cell (COS)
Specification
COS
Permeabilization
Yes - 0.1% Triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 03 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Monkey Cell (cos-7)
Specification
cos-7
Permeabilization
Yes - 0.1% Triton X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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