Anti-Tissue Factor antibody [TF9-10H10] (ab17375)

Overview

  • Product name
    Anti-Tissue Factor antibody [TF9-10H10]
    See all Tissue Factor primary antibodies
  • Description
    Mouse monoclonal [TF9-10H10] to Tissue Factor
  • Specificity
    The clone number has been updated from (2K1) to (TF9-10H10) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human tissue factor apoprotein (47kD)

  • Epitope
    Epitope is within the extracellular domain. Specific for epitope locus I.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. We would recommend antibody ab151748 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab17375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/200.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • Sequence similarities
    Belongs to the tissue factor family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD142 antibody
    • CD142 antigen antibody
    • Coagulation factor III (thromboplastin tissue factor) antibody
    • Coagulation factor III antibody
    • F3 antibody
    • FLJ17960 antibody
    • TF antibody
    • TF_HUMAN antibody
    • TFA antibody
    • Thromboplastin antibody
    • Tissue factor antibody
    see all

Anti-Tissue Factor antibody [TF9-10H10] images

  • ab17375 staining Tissue Factor by Immunocytochemistry/ Immunofluorescence in mouse BxPC-3 pancreatic adenocarcinoma cells. Cells were fixed with paraformaldehyde and blocking with 1% BSA and 1% goat serum was performed for 1 hour at 240C. Samples were incubated with primary antibody (1/200: in 1% BSA in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody.  

    See Abreview

  • ab17375 staining Tissue Factor in human nasopharynx tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0, 100°C for 20 minutes. Samples were then incubated with the primary antibody at a 1/200 dilution for 20 minutes at 25°C. An HRP-conjugated goat anti-mouse/rabbit polyclonal was used undiluted as the secondary antibody.

    See Abreview

References for Anti-Tissue Factor antibody [TF9-10H10] (ab17375)

This product has been referenced in:
  • Cugno M  et al. Enhanced tissue factor expression by blood eosinophils from patients with hypereosinophilia: a possible link with thrombosis. PLoS One 9:e111862 (2014). WB ; Human . Read more (PubMed: 25375118) »
  • Liu X  et al. Elevated immunoreactivity to tissue factor and its association with dysmenorrhea severity and the amount of menses in adenomyosis. Hum Reprod 26:337-45 (2011). IHC-P ; Human . Read more (PubMed: 21115504) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us. I would like to reassure you the quality of these antibodies may not have been affected by remaining at 4C during two weeks.

We recently undertook an in-depth study examining storage conditions and antibody perfo...

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I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued three free of charge replacements. To check the status of the order please contact our Customer Service team...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (nasopharynx)
Specification
nasopharynx
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 9.0, 20 min, 100C
Username

Abcam user community

Verified customer

Submitted Dec 17 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (CB17/SCID BxPC-3 xenograft)
Specification
CB17/SCID BxPC-3 xenograft
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
1% BSA, 1% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Username

Dr. Jennifer Dembinski

Verified customer

Submitted Mar 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human umbilical vein endothelial cells)
Loading amount
30 µg
Specification
Human umbilical vein endothelial cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 17 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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