Anti-Tissue Factor antibody [TF9-10H10] (ab35807)

Overview

  • Product name
    Anti-Tissue Factor antibody [TF9-10H10]
    See all Tissue Factor primary antibodies
  • Description
    Mouse monoclonal [TF9-10H10] to Tissue Factor
  • Specificity
    This antibody reacts with reduced and native non reduced Tissue Factor. We have data to indicate that this antibody may not cross react with Rabbit. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: ELISA, ICC/IF, WB, Flow Cyt, Dot blotmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Purified native apoprotein of human Tissue Factor (47 kDa).

  • Epitope
    This antibody recognises an epitope within the extracellular domain, locus 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab35807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
WB
Flow Cyt
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    ELISA: 1/500 - 1/5000.
    In ELISA this antibody is reactive agaisnt recombinant human tissue factor.
    Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 33 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
    • Sequence similarities
      Belongs to the tissue factor family.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD142 antibody
      • CD142 antigen antibody
      • Coagulation factor III (thromboplastin tissue factor) antibody
      • Coagulation factor III antibody
      • F3 antibody
      • FLJ17960 antibody
      • TF antibody
      • TF_HUMAN antibody
      • TFA antibody
      • Thromboplastin antibody
      • Tissue factor antibody
      see all

    References

    ab35807 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
    Sample
    Rhesus monkey Tissue sections (Aorta)
    Specification
    Aorta
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 03 2014

    Thank you for your inquiry. Please see the answer to your questions below: 1.) I can confirm that ab17375 (Anti-Tissue Factor antibody [2K1]) is derived from supernatant from hybridoma cells whereas ab35807 (Anti-Tissue Factor antibody ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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