Anti-Tissue Factor antibody [TF9-10H10] (ab35807)

Overview

  • Product nameAnti-Tissue Factor antibody [TF9-10H10]
    See all Tissue Factor primary antibodies
  • Description
    Mouse monoclonal [TF9-10H10] to Tissue Factor
  • SpecificityThis antibody reacts with reduced and native non reduced Tissue Factor. We have data to indicate that this antibody may not cross react with Rabbit. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, Flow Cyt, Dot blotmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Purified native apoprotein of human Tissue Factor (47 kDa).

  • EpitopeThis antibody recognises an epitope within the extracellular domain, locus 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab35807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
WB
Flow Cyt
Dot blot
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/500 - 1/5000.
    In ELISA this antibody is reactive agaisnt recombinant human tissue factor.
    Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 33 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInitiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
    • Sequence similaritiesBelongs to the tissue factor family.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD142 antibody
      • CD142 antigen antibody
      • Coagulation factor III (thromboplastin tissue factor) antibody
      • Coagulation factor III antibody
      • F3 antibody
      • FLJ17960 antibody
      • TF antibody
      • TF_HUMAN antibody
      • TFA antibody
      • Thromboplastin antibody
      • Tissue factor antibody
      see all

    References for Anti-Tissue Factor antibody [TF9-10H10] (ab35807)

    ab35807 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
    Sample Rhesus monkey Tissue sections (Aorta)
    Specification Aorta
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 03 2014

    Thank you for your inquiry. Please see the answer to your questions below: 1.) I can confirm that ab17375 (Anti-Tissue Factor antibody [2K1]) is derived from supernatant from hybridoma cells whereas ab35807 (Anti-Tissue Factor antibody ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"