Tissue Factor (CD142) Human ELISA Kit (ab108903)

Overview

  • Product nameTissue Factor (CD142) Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7%
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma, Cell culture extracts, Tissue
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 20 pg/ml
  • Range
    12.5 pg/ml - 400 pg/ml
  • Recovery

    97.5 %

  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Tissue Factor (CD142) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor concentrations in plasma, serum, tissue extracts and cell culture supernatants.

    A Tissue Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Tissue Factor (CD142) Human ELISA Kit images

  • Representative Standard Curve using ab108903.

Protocols

References for Tissue Factor (CD142) Human ELISA Kit (ab108903)

ab108903 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"