Tissue Factor (CD264) Human ELISA Kit (ab108903)
- Product nameTissue Factor (CD264) Human ELISA KitSee all Tissue Factor kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 4.9%
- Tests1 x 96 well plate
- Sample typeSerum, Plasma, Cell culture extracts, Tissue
- Assay typeSandwich (quantitative)
- Sensitivity= 20 pg/ml
- Range12.5 pg/ml - 400 pg/ml
- Assay time5h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Tissue Factor (CD264) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor concentrations in plasma, serum, tissue extracts and cell culture supernatants.
A Tissue Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Biotinylated Human Tissue Factor Antibody 1 x 80µl 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Tissue Factor Microplate (12 x 8 well strips) 1 unit Tissue Factor Standard 1 vial
- Research Areas
- Coagulation factor III
Our Abpromise guarantee covers the use of ab108903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Tissue Factor (CD264) Human ELISA Kit (ab108903)
ab108903 has not yet been referenced specifically in any publications.