Tissue Factor Pathway Inhibitor (TFPI) Human ELISA Kit (ab108904)
- Product nameTissue Factor Pathway Inhibitor (TFPI) Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 4.8%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Milk, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity= 0.3 ng/ml
- Range0.313 ng/ml - 20 ng/ml
- Assay time5h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Tissue Factor Pathway Inhibitor (TFPI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor Pathway Inhibitor concentrations in plasma, serum, milk and cell culture samples.
A Tissue Factor Pathway Inhibitor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor Pathway Inhibitor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor Pathway Inhibitor captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Biotinylated Human Tissue Factor Pathway Inhibitor Antibody 1 x 80µl 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Tissue Factor Pathway Inhibitor Microplate (12 x 8 well strips) 1 x 96 units Tissue Factor Pathway Inhibitor Standard 1 vial
- Research Areas
- SwissProt: P10646 Human
Our Abpromise guarantee covers the use of ab108904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tissue Factor Pathway Inhibitor (TFPI) Human ELISA Kit images
Representative Standard Curve using ab108904.
References for Tissue Factor Pathway Inhibitor (TFPI) Human ELISA Kit (ab108904)
ab108904 has not yet been referenced specifically in any publications.