Anti-Tissue Factor (phospho S290) antibody (ab62251)

Overview

  • Product nameAnti-Tissue Factor (phospho S290) antibody
    See all Tissue Factor primary antibodies
  • Description
    Rabbit polyclonal to Tissue Factor (phospho S290)
  • Specificityab62251 detects endogenous levels of Tissue Factor only when phosphorylated at serine 290.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human Tissue Factor around the phosphorylation site of serine 290 (E-N-SP-P-L).

  • Positive control
    • Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/2000. PubMed: 21310953

Target

  • FunctionInitiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • Sequence similaritiesBelongs to the tissue factor family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD142 antibody
    • CD142 antigen antibody
    • Coagulation factor III (thromboplastin tissue factor) antibody
    • Coagulation factor III antibody
    • F3 antibody
    • FLJ17960 antibody
    • TF antibody
    • TF_HUMAN antibody
    • TFA antibody
    • Thromboplastin antibody
    • Tissue factor antibody
    see all

Anti-Tissue Factor (phospho S290) antibody images

  • ab62251 at 1/50 - 1/100 dilution staining Tissue Factor in Human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • Anti-Tissue Factor (phospho S290) antibody (ab62251) at 1/2000 dilution + whole cell lysate prepared from transfected human endothelial cells expressing the human full length Tissue Factor (phospho S290) (treated with PAR2-agonist peptide for 90 minutes) at 20 µg/ml

    Secondary
    Goat anti-rabbit IgG-HRP at 1/1000 dilution

    Observed band size : 46 kDa (why is the actual band size different from the predicted?)

    Image courtesy of Dr C Ettelaie by Abreview.

    See Abreview

References for Anti-Tissue Factor (phospho S290) antibody (ab62251)

This product has been referenced in:
  • Collier ME & Ettelaie C Regulation of the incorporation of tissue factor into microparticles by serine phosphorylation of the cytoplasmic domain of tissue factor. J Biol Chem 286:11977-84 (2011). WB ; Human . Read more (PubMed: 21310953) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Transfected endothelial cells)
Loading amount 20 µg
Specification Transfected endothelial cells
Treatment PAR2-agonist peptide for 90 min
Gel Running Conditions Reduced Denaturing (12)
Blocking step TBST as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 0.05% · Temperature: 20°C
Username

Dr. C Ettelaie

Verified customer

Submitted Mar 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"