Tissue type Plasminogen Activator Human Chromogenic Activity Assay Kit (ab108905)

Overview

  • Product nameTissue type Plasminogen Activator Human Chromogenic Activity Assay Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Plasma
  • Assay typeQuantitative
  • Sensitivity
    0.03 IU/ml
  • Range
    0.039 IU/ml - 40 IU/ml
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Tissue type Plasminogen Activator (tPA) is a 68 kDa serine protease that converts the zymogen plasminogen into the active serine protease plasmin which digests fibrin and induces the dissolution of fibrin clots. Tissue type Plasminogen Activator is synthesized by endothelial cells in normal blood vessels and displays relatively high affinity for fibrin, suggesting that it functions predominately in physiological thrombolysis in vivo. High level of Tissue type Plasminogen Activator is a good prognostic marker for breast cancer. On the other hand, gastrointestinal cancer is accompanied by a decrease in Tissue type Plasminogen Activator.

     

    ab108905 Tissue type Plasminogen Activator Human Chromogenic Activity Assay kit is developed to determine Human Tissue type Plasminogen Activator activity in cell culture supernatants and plasma. The assay measures the ability of Tissue type Plasminogen Activator to activate the plasminogen to plasmin in coupled or indirect assays that contain Tissue type Plasminogen Activator, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the Tissue type Plasminogen Activator enzymatic activity.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • FunctionConverts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration.
  • Tissue specificitySynthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • Involvement in diseaseIncreased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • DomainBoth FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • Post-translational
    modifications
    The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Alternative names
    • PLAT
    • t-PA
    • T-plasminogen activator
    • Tissue-type plasminogen activator chain B
    • tPA
    • TPA_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Tissue type Plasminogen Activator Human Chromogenic Activity Assay Kit images

  • tPA measured in biological fluids showing activity (IU) per mL of tested sample

  • Time course of tPA measured in biological fluids showing optical densities

  • Standard curve (colorimetric) : mean of duplicates (+/-SD) with background readings subtracted

  • Change of absorbance per min. of various concentrations of tPA standard

  • O.D's of various dilutions of human plasma after 20hrs using ab108905

     

  • Tissue type Plasminogen Activator Chromogenic Activity Standard curve

Protocols

References for Tissue type Plasminogen Activator Human Chromogenic Activity Assay Kit (ab108905)

ab108905 has not yet been referenced specifically in any publications.

Product Wall

Mouse plasma testing

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This kit works fine for mouse plasma.
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Abcam user community

Verified customer

Submitted Mar 18 2016

Unfortunately we do not sell components from this kit separately.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"