![Immunohistochemistry (Frozen sections) - Titin antibody [T11] (ab7034) image](#ab7034_1.jpg)
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Products:Epigenetics and Nuclear Signaling >> Chromosome Structure >> Chromosome
Overview
Product name
Anti-Titin antibody [T11]
See all Titin products (2) ...
Description
Mouse monoclonal [T11] to Titin
Specificity
Monoclonal Anti-Titin reacts only with skeletal and cardiac muscle, but not with smooth muscle or different non-muscle tissues and cultured cells. The antibody localizes titin (connectin) in skeletal and heart muscle of a wide variety of species from cold-blooded vertebrates to human. The product does not cross-react with nebulin.
Tested applications
WB, IHC-Fr, IF, Immunomicroscopy, Dot Blot, Electron Microscopymore details
Cross reactivity
Reacts with
Rat, Chicken
Predicted to work with
Rabbit, Human, Pig, Fish, a wide range of other species
Does not react with
Mouse, Cow
Immunogen
Full length native protein (purified) (Chicken breast muscle).
Positive control
rat skeletal muscle, pig skeletal muscle, chicken heart muscle
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Primary antibody notes
Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors. By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs. In immunoblotting, using total extracts of chicken breast muscle, the antibody reacts specifically with both bands of the titin double and shows no reaction with nebulin.
Clonality
Monoclonal
Clone number
T11
Isotype
IgG2b
Research areas
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Actins
Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Epigenetics and Nuclear Signaling >> Chromosome Structure >> Chromosome
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Immunohistochemistry (Frozen sections) - Titin antibody [T11] (ab7034)
![Immunohistochemistry (Frozen sections) - Titin antibody [T11] (ab7034) image](/ps/datasheet/Images/7/ab7034/ab7034_1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IF
Immunomicroscopy
Dot Blot
Electron Microscopy
Application notes
Dot: Use at an assay dependent dilution.
Electron Microscopy: Use at an assay dependent dilution.
IF: 1/1000.
IHC-Fr: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 2000 kDa.
Fish skeletal muscle (Torpedo sp.) +
Toad skeletal muscle (Bufo marinus) +
Salamander skeletal muscle +
(Ambystoma tigrina)
Salamander heart muscle +
Alligator skeletal muscle +
(Alligator mississippiensis)
Pigeon skeletal muscle +
Chicken skeletal muscle +
Chicken heart muscle +
Chicken gizzard smooth muscle -
Mouse skeletal muscle -
Rat skeletal muscle +
Rat heart muscle +
Rat rhabdomyosarcoma +
Rabbit skeletal muscle +
Bovine heart muscle -
Pig skeletal muscle +
Human skeletal muscle +
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Titin is a key component in the assembly and functioning of vertebrate striated muscles. By providing connections at the level of individual microfilaments, titin contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensile properties of muscle. 8 isoforms of human titin have been reported; where isoforms 3,6,7 and 8 are expressed in cardiac muscle/tissues and isoform 4 is expressed in vertebrate skeletal muscle. Titin is also found in non-muscle cells, where it seems to play a role in chromosome condensation and chromosome segregation during interphase. Defects in titin are associated with several cardiomyopathies and muscular dystrophies.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- MPRM antibody
- Cardiomyopathy dilated 1G (autosomal dominant) antibody
- CMD1G antibody
see all
Database links
- Entrez Gene: 424126 Chicken
- Entrez Gene: 7273 Human
- Entrez Gene: 396682 Pig
- Entrez Gene: 84015 Rat
- Omim: 188840 Human
- SwissProt: Q788U5 Chicken
- SwissProt: Q8WZ42 Human
Anti-Titin antibody [T11] images:
Immunohistochemistry (Frozen sections) - Titin antibody [T11] (ab7034)
ab7034 at 1/100 staining rat soleus muscle tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with horse serum. A biotinylated horse anti-mouse IgG was used as the secondary.
This image is courtesy of an anonymous Abreview
References for Anti-Titin antibody [T11] (ab7034)
This product has been referenced in:
- Zhang Qet al. Nesprin-2 is a multi-isomeric protein that binds lamin and emerin at the nuclear envelope and forms a subcellular network in skeletal muscle. J Cell Sci 118:673-87 (2005).Read more (PubMed: 15671068) »
- Barta Jet al. Calpain-1-sensitive myofibrillar proteins of the human myocardium. Mol Cell Biochem 278:1-8 (2005).Read more (PubMed: 16180082) »
See all 6 publications for this product
Publishing research using ab7034? Please let us know so that we can cite the reference in this datasheet
![Titin antibody [T11] for IHC-Fr in Rat (7034)](/ps/Reviews/Images/ab6386_23682.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"