Titin antibody [T11] (ab7034)

Product Name 

Titin antibody [T11]

Product type 

Primary antibodies

Description 

Mouse monoclonal [T11] to Titin

Immunogen 

Full length native protein (purified) (Chicken breast muscle).

Reacts with 

Rat, Chk

Does not react with 

Ms, Cow

Predicted to react 
(species key)

Hu, Many, Fsh, Pig, Rb
(Due to sequence homology)

Specificity 

Monoclonal Anti-Titin reacts only with skeletal and cardiac muscle, but not with smooth muscle or different non-muscle tissues and cultured cells.

The antibody localizes titin (connectin) in skeletal and heart muscle of a wide variety of species from cold-blooded vertebrates to human.

The product does not cross-react with nebulin.

Tested applications 
(see key)

Dot, EM, ICC/IF, IHC-Fr, IM, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
Electron Microscopy: Use at an assay dependent dilution.
IF: 1/1000.
IHC-Fr: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 2000 kDa.

Fish skeletal muscle (Torpedo sp.) +
Toad skeletal muscle (Bufo marinus) +
Salamander skeletal muscle +
(Ambystoma tigrina)
Salamander heart muscle +
Alligator skeletal muscle +
(Alligator mississippiensis)
Pigeon skeletal muscle +
Chicken skeletal muscle +
Chicken heart muscle +
Chicken gizzard smooth muscle -
Mouse skeletal muscle -
Rat skeletal muscle +
Rat heart muscle +
Rat rhabdomyosarcoma +
Rabbit skeletal muscle +
Bovine heart muscle -
Pig skeletal muscle +
Human skeletal muscle +

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

rat skeletal muscle, pig skeletal muscle, chicken heart muscle

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Actins
Cardiovascular >> Heart >> Hypertrophy >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins

Relevance 

Titin is a key component in the assembly and functioning of vertebrate striated muscles. By providing connections at the level of individual microfilaments, titin contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensile properties of muscle. 8 isoforms of human titin have been reported; where isoforms 3,6,7 and 8 are expressed in cardiac muscle/tissues and isoform 4 is expressed in vertebrate skeletal muscle. Titin is also found in non-muscle cells, where it seems to play a role in chromosome condensation and chromosome segregation during interphase. Defects in titin are associated with several cardiomyopathies and muscular dystrophies.

Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures.

It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors.

By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues.

Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs.

In immunoblotting, using total extracts of chicken breast muscle, the antibody reacts specifically with both bands of the titin double and shows no reaction with nebulin.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

T11

Isotype 

IgG2b

Purity 

Ascites

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: Ascites

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 3.00 - 3.50 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Titin antibody [T11] images, references, products related to ab7034 and other tools.

Titin antibody [T11] images:

References for Titin antibody [T11] (ab7034)

  This product has been used in: (two most recent references)

Zhang Q et al. Nesprin-2 is a multi-isomeric protein that binds lamin and emerin at the nuclear envelope and forms a subcellular network in skeletal muscle. J Cell Sci 118:673-87 (2005). PubMed: 15671068

Barta J et al. Calpain-1-sensitive myofibrillar proteins of the human myocardium. Mol Cell Biochem 278:1-8 (2005). PubMed: 16180082

See all 6 publication references for this product.


If you publish research using ab7034 please let us know so that we can cite the reference on this datasheet.

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