The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 62 for isoform 1 , for isoform 4, 53 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The TJAP1 gene encodes a tight junction-associated protein. Incorporation of the encoded protein into tight junctions occurs at a late stage of formation of the junctions. The encoded protein may function in vesicle trafficking and localizes to the Golgi apparatus. Alternatively spliced transcript variants have been described. A related pseudogene exists on the X chromosome.
Golgi apparatus. Cell junction › tight junction. Note= Recruited to tight junctions (TJ) during late stages of maturation of the TJ complexes. Excluded from adherens junctions and desmosomes.