The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceThe TJAP1 gene encodes a tight junction-associated protein. Incorporation of the encoded protein into tight junctions occurs at a late stage of formation of the junctions. The encoded protein may function in vesicle trafficking and localizes to the Golgi apparatus. Alternatively spliced transcript variants have been described. A related pseudogene exists on the X chromosome.
Cellular localizationGolgi apparatus. Cell junction › tight junction. Note= Recruited to tight junctions (TJ) during late stages of maturation of the TJ complexes. Excluded from adherens junctions and desmosomes.