Overview

  • Product nameAnti-TJAP1 antibody
    See all TJAP1 primary antibodies
  • Description
    Rabbit polyclonal to TJAP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: EGPGTSHTEG RAWPLPSSSR PQRSPKRMGV HHLHRKDSLT QAQEQGNLLN , corresponding to C terminal amino acids 497-546 of Human TJAP1

  • Positive control
    • Placenta lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceThe TJAP1 gene encodes a tight junction-associated protein. Incorporation of the encoded protein into tight junctions occurs at a late stage of formation of the junctions. The encoded protein may function in vesicle trafficking and localizes to the Golgi apparatus. Alternatively spliced transcript variants have been described. A related pseudogene exists on the X chromosome.
  • Cellular localizationGolgi apparatus. Cell junction › tight junction. Note= Recruited to tight junctions (TJ) during late stages of maturation of the TJ complexes. Excluded from adherens junctions and desmosomes.
  • Database links
  • Alternative names
    • PILT antibody
    • Protein incorporated later into tight junctions antibody
    • tight junction associated protein 1 peripheral antibody
    • Tight junction protein 4 antibody
    • tight junction protein 4 peripheral antibody
    • TJP4 antibody
    see all

Anti-TJAP1 antibody images

  • Anti-TJAP1 antibody (ab80444) at 1 µg/ml + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TJAP1 antibody (ab80444)

This product has been referenced in:
  • Charania MA  et al. Intestinal epithelial CD98 synthesis specifically modulates expression of colonic microRNAs during colitis. Am J Physiol Gastrointest Liver Physiol 302:G1282-91 (2012). WB ; Mouse . Read more (PubMed: 22499850) »
  • Cambronne XA  et al. Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach. Proc Natl Acad Sci U S A 109:20473-8 (2012). WB . Read more (PubMed: 23184980) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"