Overview

  • Product name
  • Description
    Rabbit polyclonal to TJP2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 993-1042 (EPQKAPSRPYQDTRGSYGSDAEEEEYRQQLSEHSKRGYYGQSARYRDTE L) of Human TJP2 (NP_963923).

  • Positive control
    • Jurkat cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 134 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

Target

  • Function
    Plays a role in tight junctions and adherens junctions.
  • Tissue specificity
    This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.
  • Involvement in disease
    Defects in TJP2 are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
  • Sequence similarities
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • Cellular localization
    Cell junction > adherens junction. Cell membrane. Cell junction > tight junction. Nucleus. Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.
  • Information by UniProt
  • Database links
  • Alternative names
    • C9DUPq21.11 antibody
    • DFNA51 antibody
    • DUP9q21.11 antibody
    • Friedreich ataxia region gene X104 (tight junction protein ZO-2) antibody
    • MGC26306 antibody
    • PFIC4 antibody
    • Tight junction protein 2 antibody
    • Tight junction protein ZO 2 antibody
    • Tight junction protein ZO-2 antibody
    • TJP2 antibody
    • X104 antibody
    • ZO 2 antibody
    • ZO-2 antibody
    • ZO2 antibody
    • ZO2_HUMAN antibody
    • Zona occludens 2 antibody
    • Zona occludens protein 2 antibody
    • Zonula occludens protein 2 antibody
    see all

Anti-TJP2 antibody images

  • Anti-TJP2 antibody (ab81679) at 1 µg/ml ( in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 134 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TJP2 antibody (ab81679)

ab81679 has not yet been referenced specifically in any publications.

Product Wall

Western blot

Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Mouse Cell lysate - whole cell (C2C12 cells and Mouse Embryonic Fibroblasts)
Specification
C2C12 cells and Mouse Embryonic Fibroblasts
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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Verified customer

Submitted Jun 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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