Overview

  • Product nameAnti-TKTL1 antibody
    See all TKTL1 primary antibodies
  • Description
    Mouse monoclonal to TKTL1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-597 of Human TKTL1

Properties

Applications

Our Abpromise guarantee covers the use of ab56469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein

Target

  • Tissue specificityExpressed in fetal and adult heart, brain, lung, liver, and kidney, and in adult placenta, skeletal muscle and pancreas. Up-regulated in various epithelial tumors.
  • Sequence similaritiesBelongs to the transketolase family.
  • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic and to a lesser extent also nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • TK 2 antibody
    • TK2 antibody
    • TKR antibody
    • TKT 2 antibody
    • TKT2 antibody
    • TKTL1 antibody
    • TKTL1_HUMAN antibody
    • Transketolase 2 antibody
    • Transketolase like 1 antibody
    • Transketolase like protein 1 antibody
    • Transketolase related protein antibody
    • transketolase-like 1 antibody
    • Transketolase-like protein 1 antibody
    • Transketolase-related protein antibody
    • Transketolase2 antibody
    see all

Anti-TKTL1 antibody images

  • Western blot against tagged recombinant protein immunogen using ab56469 TKTL1 antibody at 1ug/ml. Predicted band size of immunogen is 92 kDa
  • ICC/IF image of ab56469 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56469, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TKTL1 antibody (ab56469)

ab56469 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HMEC, MCF-10A, LN229, MDA-MB231 cell lines)
Loading amount 10 µg
Specification HMEC, MCF-10A, LN229, MDA-MB231 cell lines
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. barbara chaneton

Verified customer

Submitted Jan 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"