The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 28 kDa. The detection limit for recombinant Human TL1A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 0.5 - 2 µg/ml. ab109829 in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TL1A (using 100 µl/well antibody solution).
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to TL1A (Biotin) (ab84233). ab109829 in conjunction with ab84233 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TL1A (using 100 µl/well antibody solution).
FunctionReceptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.
Tissue specificitySpecifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon.
Sequence similaritiesBelongs to the tumor necrosis factor family.