• Product name
  • Description
    Mouse polyclonal to TLE 1
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human TLE 1 (NP_005068.2)

  • Positive control
    • TLE 1 transfected 293T cell lysate, Human liver tissue lysate and Hela cells.



Our Abpromise guarantee covers the use of ab89782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa.
ICC/IF Use a concentration of 10 µg/ml.


  • Function
    Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
  • Tissue specificity
    In all tissues examined, mostly in brain, liver and muscle.
  • Sequence similarities
    Belongs to the WD repeat Groucho/TLE family.
    Contains 6 WD repeats.
  • Domain
    WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.
  • Post-translational
    Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.
    Ubiquitinated by XIAP/BIRC4.
  • Cellular localization
    Nucleus. Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.
  • Information by UniProt
  • Database links
  • Alternative names
    • E(Sp1) homolog antibody
    • Enhancer of split groucho 1 antibody
    • Enhancer of split groucho like protein 1 antibody
    • Enhancer of split groucho-like protein 1 antibody
    • ESG antibody
    • ESG1 antibody
    • GRG1 antibody
    • TLE1 antibody
    • TLE1_HUMAN antibody
    • Transducin like enhancer of split 1 (E(sp1) homolog Drosophila) antibody
    • Transducin like enhancer of split 1 antibody
    • Transducin like enhancer of split 1 homolog of Drosophila E(sp1) antibody
    • Transducin like enhancer protein 1 antibody
    • Transducin-like enhancer protein 1 antibody
    see all


  • Anti-TLE 1 antibody (ab89782) at 1 µg/ml + human liver tissue lysate at 50 µg

    Predicted band size: 83 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-TLE 1 antibody (ab89782) at 1 µg/ml

    Lane 1 : TLE 1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 83 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)

  • ab89782, at 10µg/ml, staining TLE 1 in HeLa cells by Immunofluorescence.


This product has been referenced in:
  • Brunquell C  et al. TLE1 Is an Anoikis Regulator and Is Downregulated by Bit1 in Breast Cancer Cells. Mol Cancer Res 10:1482-95 (2012). WB . Read more (PubMed: 22952044) »

See 1 Publication for this product

Customer reviews and Q&As

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab89782.

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I am sorry that you are experiencing issues with ab89782 in western blot. Under our Abpromise, we do guarantee this antibody, for 6 months, to work in western blot against human samples.

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Thank you very much for your interest in ab89782.

To our knowledge, ab89782 has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab89782 now, test it inIHC and submit feedback to us in the form of an...

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