The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.
Tissue specificityIn all tissues examined, mostly in heart, brain, and muscle.
Sequence similaritiesBelongs to the WD repeat Groucho/TLE family. Contains 6 WD repeats.