Overview

  • Product name
  • Description
    Rabbit polyclonal to TLE3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human TLE3 aa 173-215.
    Sequence:

    GSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHR


    Database link: Q04726

  • Positive control
    • Human vulva/anal skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab185508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.
  • Tissue specificity
    Placenta and lung.
  • Sequence similarities
    Belongs to the WD repeat Groucho/TLE family.
    Contains 7 WD repeats.
  • Post-translational
    modifications
    Ubiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E(sp1) homolog Drosophila antibody
    • Enhancer of split groucho 3 antibody
    • Enhancer of split groucho-like protein 3 antibody
    • ESG 3 antibody
    • ESG antibody
    • ESG3 antibody
    • FLJ39460 antibody
    • GRG 3 antibody
    • GRG3 antibody
    • HsT18976 antibody
    • KIAA1547 antibody
    • TLE 3 antibody
    • Tle3 antibody
    • TLE3_HUMAN antibody
    • Transducin like enhancer of split 3 antibody
    • Transducin like enhancer of split 3 homolog of Drosophila E(sp1) antibody
    • Transducin like enhancer protein 3 antibody
    • transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) antibody
    • Transducin-like enhancer protein 3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human vulva/anal skin tissue, labeling TLE3 using ab185508 at a 1/500 dilution.

  • Anti-TLE3 antibody (ab185508) + HeLa nuclear extract

    Predicted band size: 83 kDa

  • Immunofluorescent staining of human cell line HeLa staining TLE3 with ab185508. Antibody staining is shown in green

References

ab185508 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (cortex)
Antigen retrieval step
None
Permeabilization
Yes - 0,1% Triton
Specification
cortex
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Mar 24 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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