The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
TLE4 (Transducin-like enhancer protein 4) is a member of the Groucho family of transcriptional corepressors and inhibits the transcriptional activation mediated by Pax5 and by beta-catenin and TCF family members in Wnt signaling.
All lanes : Anti-TLE4 antibody (ab64833) at 1/500 dilution
Lane 1 : Jurkat cell extract at 30 µg Lane 2 : 293 cell extract at 30 µg Lane 3 : HuvEc cell extract at 30 µg Lane 4 : HuvEc cell extract immunising peptide at 10 µg
Predicted band size: 84 kDa Observed band size: 84 kDa
Immunohistochemistry (Frozen sections) - TLE4 antibody (ab64833)This image is courtesy of an anonymous Abreview
ab64833 staining TLE4 in mouse (E16.5) embryonic brain tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde, permeabilized in 0.3% Triton X-100 and blocked in 0.2% Gelatin for 1 hour at 25°C. The primary antibody was diluted 1/100 and incubated with sample for 24 hours at 4°C. A Cy5® conjugated donkey polyclonal to rabbit IgG, diluted 1/500 was used as secondary.