Overview

  • Product nameAnti-TLE6 antibody
    See all TLE6 primary antibodies
  • Description
    Mouse monoclonal to TLE6
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant full length Human TLE6 protein (AAH20206) with tag.

  • Positive control
    • Recombinant tagged full length human TLE6 protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab76858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 50 kDa.

    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAs a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions.
    • Sequence similaritiesBelongs to the WD repeat Groucho/TLE family.
      Contains 7 WD repeats.
    • Cellular localizationCytoplasm. In the subcortical cytoplasm of early embryos from the 1-cell to the blastocyst stages. From the 2-cell stage, still detected in the subcortex, but excluded from cell-cell contact regions.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ14009 antibody
      • GRG6 antibody
      • Groucho related protein 6 antibody
      • MGC14966 antibody
      • TLE 6 antibody
      • TLE6 antibody
      • TLE6_HUMAN antibody
      • Transducin like enhancer of split 6 (E(sp1) homolog Drosophila) antibody
      • Transducin like enhancer of split 6 antibody
      • Transducin like enhancer protein 6 antibody
      • Transducin-like enhancer protein 6 antibody
      see all

    Anti-TLE6 antibody images

    • Anti-TLE6 antibody (ab76858) at 1 µg/ml + Recombinant tagged full length human TLE6 protein at 0.1 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 50 kDa
      Observed band size : 76 kDa (why is the actual band size different from the predicted?)

    References for Anti-TLE6 antibody (ab76858)

    ab76858 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76858.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"