Overview

  • Product name
  • Description
    Mouse monoclonal to TLK2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-126 of Human TLK2

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab56816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 88 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.
  • Tissue specificity
    Widely expressed. Present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. Also found in adult cell lines.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC44450 antibody
    • PKU alpha antibody
    • PKU-alpha antibody
    • Serine/threonine kinase antibody
    • Serine/threonine protein kinase tousled like 2 antibody
    • Serine/threonine-protein kinase tousled-like 2 antibody
    • TLK 2 antibody
    • tlk2 antibody
    • TLK2_HUMAN antibody
    • Tousled like kinase 2 antibody
    • Tousled-like kinase 2 antibody
    see all

Anti-TLK2 antibody images

  • TLK2 antibody (ab56816) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon tissue.


  • Predicted band size : 88 kDa
    TLK2 antibody (ab56816) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab56816 staining TLK2 in U2OS human osteosarcoma cell line by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using 0.5% Triton/PBS for 5 minutes, blocked with 10% serum for 1 hour at room temperature and then incubated with ab56816 at a 1/100 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/500 dilution.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab56816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56816, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-TLK2 antibody (ab56816)

ab56816 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS human osteosarcoma cell line)
Specification
U2OS human osteosarcoma cell line
Fixative
Formaldehyde
Permeabilization
Yes - triton 0,5% in PBS for 5 min
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Verified customer

Submitted Feb 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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