The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 88 kDa.
Use a concentration of 5 µg/ml.
Use at an assay dependent concentration.
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionRapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.
Tissue specificityWidely expressed. Present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. Also found in adult cell lines.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
TLK2 antibody (ab56816) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon tissue.
Western blot - TLK2 antibody (ab56816)
Predicted band size : 88 kDa TLK2 antibody (ab56816) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Immunocytochemistry/ Immunofluorescence - Anti-TLK2 antibody (ab56816)Image courtesy of an anonymous Abreview.
ab56816 staining TLK2 in U2OS human osteosarcoma cell line by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permeabilized using 0.5% Triton/PBS for 5 minutes, blocked with 10% serum for 1 hour at room temperature and then incubated with ab56816 at a 1/100 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/500 dilution.
Overlay histogram showing HeLa cells stained with ab56816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56816, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-TLK2 antibody (ab56816)
has not yet been referenced specifically in any publications.
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