• Product nameAnti-TLK2 antibody
    See all TLK2 primary antibodies
  • Description
    Mouse monoclonal to TLK2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-126 of Human TLK2



Our Abpromise guarantee covers the use of ab56816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 88 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionRapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.
  • Tissue specificityWidely expressed. Present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. Also found in adult cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC44450 antibody
    • PKU alpha antibody
    • PKU-alpha antibody
    • Serine/threonine kinase antibody
    • Serine/threonine protein kinase tousled like 2 antibody
    • Serine/threonine-protein kinase tousled-like 2 antibody
    • TLK 2 antibody
    • tlk2 antibody
    • TLK2_HUMAN antibody
    • Tousled like kinase 2 antibody
    • Tousled-like kinase 2 antibody
    see all

Anti-TLK2 antibody images

  • TLK2 antibody (ab56816) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon tissue.

  • Predicted band size : 88 kDa
    TLK2 antibody (ab56816) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab56816 staining TLK2 in U2OS human osteosarcoma cell line by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using 0.5% Triton/PBS for 5 minutes, blocked with 10% serum for 1 hour at room temperature and then incubated with ab56816 at a 1/100 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/500 dilution.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab56816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56816, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-TLK2 antibody (ab56816)

ab56816 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS human osteosarcoma cell line)
Specification U2OS human osteosarcoma cell line
Fixative Formaldehyde
Permeabilization Yes - triton 0,5% in PBS for 5 min
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

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Submitted Feb 06 2012