Overview

  • Product nameAnti-TLK2 antibody
    See all TLK2 primary antibodies
  • Description
    Rabbit polyclonal to TLK2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 722 and 772 of human TLK2 (EAW94344.1)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab74335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 88 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionRapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.
  • Tissue specificityWidely expressed. Present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. Also found in adult cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC44450 antibody
    • PKU alpha antibody
    • PKU-alpha antibody
    • Serine/threonine kinase antibody
    • Serine/threonine protein kinase tousled like 2 antibody
    • Serine/threonine-protein kinase tousled-like 2 antibody
    • TLK 2 antibody
    • tlk2 antibody
    • TLK2_HUMAN antibody
    • Tousled like kinase 2 antibody
    • Tousled-like kinase 2 antibody
    see all

Anti-TLK2 antibody images

  • All lanes : Anti-TLK2 antibody (ab74335) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 5 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 88 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of TLK2 by Western Blot of Immunprecipitate.
    ab74335 at 1µg/ml staining TLK2 in 293 whole cell lysate immunoprecipitated using ab74335 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-TLK2 antibody (ab74335)

ab74335 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"