Anti-TLR1 antibody [GD2.F4] (FITC) (ab59702)

Overview

  • Product nameAnti-TLR1 antibody [GD2.F4] (FITC)
    See all TLR1 primary antibodies
  • Description
    Mouse monoclonal [GD2.F4] to TLR1 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis antibody reacts with human TLR1. It does not recognize TLR2, TLR4 or TLR4 in association with MD2.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human TLR1.

Properties

Applications

Our Abpromise guarantee covers the use of ab59702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionParticipates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityUbiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Cellular localizationCell membrane. Cytoplasmic vesicle > phagosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD281 antibody
    • CD281 antigen antibody
    • KIAA0012 antibody
    • LPRS5 antibody
    • rsc786 antibody
    • TIL antibody
    • TIL. LPRS5 antibody
    • Tlr1 antibody
    • TLR1_HUMAN antibody
    • Toll (Drosophila) homolog antibody
    • Toll like receptor 1 antibody
    • Toll-like receptor 1 antibody
    • Toll/interleukin-1 receptor-like protein antibody
    see all

Anti-TLR1 antibody [GD2.F4] (FITC) images

  • Overlay histogram showing THP1 cells stained with ab59702 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59702, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 FITC (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-TLR1 antibody [GD2.F4] (FITC) (ab59702)

ab59702 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMCs)
Specification PBMCs
Preparation Cell harvesting/tissue preparation method: peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation for monocytes
Sample buffer: PBS with human IgG (Fc-block)
Fixation no fixation
Permeabilization No
Gating Strategy monocyte gate
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Submitted Jan 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"