Overview

  • Product nameAnti-TLR11 antibody
    See all TLR11 primary antibodies
  • Description
    Rabbit polyclonal to TLR11
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    CEQLKRRLSKAGQERD

    , corresponding to C terminal amino acids 911-926 of Mouse TLR11

  • Positive control
    • Mouse kidney tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab47097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 21030601
Flow Cyt Use 0.5-1µg for 106 cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).

Target

  • RelevanceToll like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR11 is one of three mouse TLRs that lack a human ortholog. It is activated specifically by uropathogenic bacteria, and mice lacking TLR11 showed a much greater susceptability to uropathogenic infections, indicating a potentially important role for TLR11 in preventing infections in the urogenital system.
  • Cellular localizationType I membrane protein
  • Database links
  • Alternative names
    • hgnc31753 antibody
    • Toll like receptor 11 antibody

Anti-TLR11 antibody images

  • All lanes : Anti-TLR11 antibody (ab47097) at 3 µg/ml

    Lane 1 : mouse testis lysate
    Lane 2 : mouse testis lysate with blocking peptide
    Lane 3 : rat testis lysate
    Lane 4 : rat testis lysate with blocking peptide


    Predicted band size : 100 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa (possible degradation product),55 kDa. We are unsure as to the identity of these extra bands.
  • Intracellular flow analysis of TLR11 in Balb/c mouse splenocytes using ab47097 at 0.5 ug/106 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control; red represents anti-TLR11 antibody. Goat anti-rabbit PE was used as secondary antibody.

References for Anti-TLR11 antibody (ab47097)

This product has been referenced in:
  • Chen Q  et al. Toll-like receptor 11-initiated innate immune response in male mouse germ cells. Biol Reprod 90:38 (2014). Mouse . Read more (PubMed: 24403550) »
  • Hageman RS  et al. Uncovering genes and regulatory pathways related to urinary albumin excretion. J Am Soc Nephrol 22:73-81 (2011). IHC-P ; Mouse . Read more (PubMed: 21030601) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Liver)
Loading amount 20 µg
Specification Liver
Gel Running Conditions Non-reduced Denaturing (7,5% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Oct 17 2012

Thank you for contacting us. We apologize for the error. The label should read as anti-TLR11 antibody. We unfortunately do not have any anti TLR12 antibody available so this product can't be TLR12. Please use the antibody as normal if in case you a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"