Overview

  • Product name
  • Description
    Goat polyclonal to TLR2
  • Specificity
    Peptide sequence is < 50 % identical to other human TLR receptors in this region.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    CLEIDASDLQSYEPKSLKSIQNVSHLI

    , corresponding to amino acids 179-204 of Human TLR2.

  • Positive control
    • Human spleen sections or human peripheral blood

Properties

Applications

Our Abpromise guarantee covers the use of ab1655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250.
IHC-Fr Use at an assay dependent dilution. PubMed: 20534434
ICC/IF 1/500.
ELISA 1/75000.

Target

  • Function
    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • Tissue specificity
    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

Anti-TLR2 antibody images

  • Immunohistochemistry on human spleen at 1:250 dilution.
  • ab1655 staining TLR2 in murine brain tissue by Immunohistochemistry (Frozen sections).
    Frozen sections were mounted on glass slides, postfixed in ice-cold acetone, blocked with 1% BSA and 3% normal donkey serum, and incubated with ab1655 at 4°C. A FITC-conjugated secondary antibody was used to detect the primary antibody. Counterstained with DAPI.

References for Anti-TLR2 antibody (ab1655)

This product has been referenced in:
  • Shaw JL  et al. Chlamydia trachomatis infection increases fallopian tube PROKR2 via TLR2 and NF?B activation resulting in a microenvironment predisposed to ectopic pregnancy. Am J Pathol 178:253-60 (2011). IHC-P ; Human . Read more (PubMed: 21224062) »
  • Sloane JA  et al. Hyaluronan blocks oligodendrocyte progenitor maturation and remyelination through TLR2. Proc Natl Acad Sci U S A 107:11555-60 (2010). ICC/IF, IHC-Fr ; Mouse . Read more (PubMed: 20534434) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cynomolgus Monkey Tissue lysate - whole (macaque retina)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
200 µg
Specification
macaque retina
Blocking step
Milk as blocking agent for 23 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Dec 06 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Muller Cell Line MIO-M1)
Permeabilization
Yes - 0.1% TritonX-100
Specification
Muller Cell Line MIO-M1
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 01 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (lymphonode)
Antigen retrieval step
Other
Permeabilization
No
Specification
lymphonode
Blocking step
TBS albumin 2% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Formaldehyde
Username

Pamela Martinez

Verified customer

Submitted Jul 21 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (macaque retina tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
macaque retina tissue
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 10 2016

Thank you for your enquiry. Unfortunately we do not sell the blocking peptide for this antiserum. According to the EXPASY protein database (Swiss-Prot) TLR2 is "highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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