The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 20534434
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
Belongs to the Toll-like receptor family. Contains 14 LRR (leucine-rich) repeats. Contains 1 TIR domain.
Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
Toll/interleukin 1 receptor like protein 4 antibody
Toll/interleukin receptor like protein 4 antibody
Toll/interleukin-1 receptor-like protein 4 antibody
Anti-TLR2 antibody images
- TLR2 antibody (ab1655)
Immunohistochemistry on human spleen at 1:250 dilution.
Immunohistochemistry (Frozen sections) - TLR2 antibody (ab1655)Image from Sloane JA et al, Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11555-60. Epub 2010 Jun 3, Fig 4. DOI 10.1073/pnas.1006496107
ab1655 staining TLR2 in murine brain tissue by Immunohistochemistry (Frozen sections). Frozen sections were mounted on glass slides, postfixed in ice-cold acetone, blocked with 1% BSA and 3% normal donkey serum, and incubated with ab1655 at 4°C. A FITC-conjugated secondary antibody was used to detect the primary antibody. Counterstained with DAPI.
Shaw JL et al. Chlamydia trachomatis infection increases fallopian tube PROKR2 via TLR2 and NF?B activation resulting in a microenvironment predisposed to ectopic pregnancy. Am J Pathol178:253-60 (2011).
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Sloane JA et al. Hyaluronan blocks oligodendrocyte progenitor maturation and remyelination through TLR2. Proc Natl Acad Sci U S A107:11555-60 (2010).
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