The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 6 µg/ml. Predicted molecular weight: 90 kDa.
Use 2.5µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionCooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
Tissue specificityHighly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
Sequence similaritiesBelongs to the Toll-like receptor family. Contains 14 LRR (leucine-rich) repeats. Contains 1 TIR domain.
Post-translational modificationsGlycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
Cell surface flow cytometric analysis of TLR2, using ab109810. Thyoglycollate-elicited peritoneal macrophages from C57BL/6 mice were tested with 2.5 µg of isotype control antibody (green) and 2.5 µg of TLR2 antibody (red), and APC-conjugated secondary.
References for Anti-TLR2 antibody [IMG11G5] (ab109810)
has not yet been referenced specifically in any publications.
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