Overview

  • Product nameAnti-TLR2 antibody [IMG11G5]
    See all TLR2 primary antibodies
  • Description
    Rat monoclonal [IMG11G5] to TLR2
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide against a portion between amino acids 400-450 of Mouse TLR2 (UniProt ID: Q9QUN7).

  • Positive control
    • Mouse RAW cell lysate; Thyoglycollate-elicited peritoneal macrophages from C57BL/6 mice

Properties

Applications

Our Abpromise guarantee covers the use of ab109810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 4 - 6 µg/ml. Predicted molecular weight: 90 kDa.
Flow Cyt Use 2.5µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • Tissue specificityHighly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

Anti-TLR2 antibody [IMG11G5] images

  • Anti-TLR2 antibody [IMG11G5] (ab109810) at 5 µg/ml + Mouse RAW cell lysate at 40 µg

    Secondary
    Goat anti-rat Ig HRP

    Predicted band size : 90 kDa
  • Cell surface flow cytometric analysis of TLR2, using ab109810.
    Thyoglycollate-elicited peritoneal macrophages from C57BL/6 mice were tested with 2.5 µg of isotype control antibody (green) and 2.5 µg of TLR2 antibody (red), and APC-conjugated secondary.

References for Anti-TLR2 antibody [IMG11G5] (ab109810)

ab109810 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"