Overview

  • Product nameAnti-TLR2 antibody [T2.5]
    See all TLR2 primary antibodies
  • Description
    Mouse monoclonal [T2.5] to TLR2
  • Tested applicationsSuitable for: Functional Studies, ICC/IF, WB, IP, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Mouse TLR2 peptide

  • Positive control
    • RAW264.7 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab16894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 25404049
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionCooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • Tissue specificityHighly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

Anti-TLR2 antibody [T2.5] images

  • Anti-TLR2 antibody [T2.5] (ab16894) at 1/100 dilution + Human lymph node tissue lysate - total protein (ab29871) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 89 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa,65 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TLR2 antibody [T2.5] (ab16894)

This product has been referenced in:
  • Patel P  et al. Early-stage treatment with Withaferin A reduces levels of misfolded superoxide dismutase 1 and extends lifespan in a mouse model of amyotrophic lateral sclerosis. Neurotherapeutics 12:217-33 (2015). WB ; Mouse . Read more (PubMed: 25404049) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (human monocytic cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification human monocytic cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Aug 31 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"