Overview

  • Product nameAnti-TLR2 antibody [T2.5] (FITC)
    See all TLR2 primary antibodies
  • Description
    Mouse monoclonal [T2.5] to TLR2 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, IP, ICC/IF, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Mouse TLR2 peptide.

  • Positive control
    • RAW264.7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab59711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
Flow Cyt Use 10µl for 106 cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP 1/50.
ICC/IF 1/50.
Functional Studies Use at an assay dependent concentration.

Target

  • FunctionCooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • Tissue specificityHighly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

Anti-TLR2 antibody [T2.5] (FITC) images

  • Overlay histogram showing THP1 cells stained with ab59711 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59711, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 FITC (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-TLR2 antibody [T2.5] (FITC) (ab59711)

This product has been referenced in:
  • Liao K  et al. Cocaine-mediated induction of microglial activation involves the ER stress-TLR2 axis. J Neuroinflammation 13:33 (2016). Flow Cyt ; Mouse . Read more (PubMed: 26860188) »
  • Selejan S  et al. Ischaemia-induced up-regulation of Toll-like receptor 2 in circulating monocytes in cardiogenic shock. Eur Heart J 33:1085-94 (2012). Flow Cyt ; Human . Read more (PubMed: 21998404) »

See all 3 Publications for this product

Product Wall

Thank you for your phone call.

The free of charge replacement antibody you requested, ab24192, will ship on order ***. This antibody has not been validated for use on frozen sections, but may be suitable for that purpose. The antibody may req...

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I have found another reference that is more encouraging:

TLR2 is constitutively expressed within the kidney and participates in ischemic renal injury through both MyD88-dependent and -independent pathways.
J Immunol. 2007 May 15;178(10)...

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Thank you for contacting us. So far, the one reference I have found that used this antibody with frozen tissue sections used acetone as the fixative, not PFA.

Kidneys were snap frozen in liquid nitrogen and stored at –70°C prior to use. For ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"