Overview

  • Product nameAnti-TLR2 antibody [TL2.1] (Biotin)
    See all TLR2 primary antibodies
  • Description
    Mouse monoclonal [TL2.1] to TLR2 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, Functional Studies, IHC-Pmore details
  • Species reactivity
    Reacts with: Dog, Human, Cynomolgus Monkey, Rhesus monkey, Marmoset (common)
  • Immunogen

    Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.

  • General notes


    Ab9101 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab9101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Functional Studies Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionCooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • Tissue specificityHighly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

Anti-TLR2 antibody [TL2.1] (Biotin) images

  • TLR2 staining on human peripheral blood mononuclear cells by using ab9101. Green: CD3+-cells; Red: CD14+ cells.

References for Anti-TLR2 antibody [TL2.1] (Biotin) (ab9101)

This product has been referenced in:
  • Flo TH  et al. Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol 69:474-81 (2001). Read more (PubMed: 11261796) »
  • Wang JE  et al. Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun 69:2402-6 (2001). Read more (PubMed: 11254600) »

See all 3 Publications for this product

Product Wall

The following article describes the use of HM2064 (monoclonal antibody against human TLR-2, clone TL2.1): Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NK...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"