Overview

  • Product nameAnti-TLR3 antibody [40C1285]
    See all TLR3 primary antibodies
  • Description
    Mouse monoclonal [40C1285] to TLR3
  • SpecificityNegative results in WB have been obtained for mouse interstine lysates (human intestine is a positive control). The protein is heavily glycosylated and the observed band size should be higher than the predicted 103kDa. The protein is found at different molecular weight in different tissues so optimization may be necessary.
  • Tested applicationsSuitable for: IHC-P, WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    VLNLTHNQLRRLPAAN

    conjugated to KLH, corresponding to amino acids 55-70 of Human TLR3

  • Positive control
    • Human intestine tissue lysate, mouse spleen

Properties

Applications

Our Abpromise guarantee covers the use of ab13915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Permeabilization of samples might be necessary and overnight incubation with the ab13915 is recommended.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa). TLR3 is a single-pass type I membrane protein. We recomend not to boil the samples, instead heat lysates at 60-70C for 10 minutes.
Flow Cyt Use 2-5µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use 2µg for 106 cells.

Target

  • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityExpressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • Involvement in diseaseDefects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • Domainds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • Post-translational
    modifications
    Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • Cellular localizationEndoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all

Anti-TLR3 antibody [40C1285] images



  • Predicted band size : 108 kDa


    Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 µg/lane human intestine tissue lysate (lane C).
  • ab13915 at 2µg / 1x106 cells staining intracellular TLR3 in human monocytes by Flow Cytometery. A PE-conjugated goat polyclonal to mouse IgG1 was used as secondary. The shaded histogram repersents monocytes without antibody; green repersents isotype control and red histogram shows staining with ab13915. 

  • ab13915 at 5ug/ml staining TLR3 in mouse spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The left image repersents staining with isotype control and right one repersents staining with ab13915.

  • ab13915 staining TLR3 in SW480, SW620 (colon cancer) and FaDu, Detroit 562 obtained from human cells by Flow Cytometry. Samples were prepared by trypsinizining and washing in PBS and centrifugation in a buffer containing 2% FBS, 0.1% Na-azide and PBS. Cells were fixed in paraformaldehyde and permeabilized in 0.2% Tween. Gating and analysis was performed by WinMDI 2.9 software. The primary antibody was diluted to 1 µg/cells (PBS, 2% FBS and 0.1% Na-azide) and incubated with sample for 30 minutes at 4°C. An FITC conjugated rabbit polyclonal to mouse IgG, diluted to 1/40, was used as secondary. Image represents FACS analysis of TLR3 protein expression in SW480 (A), SW620 (B), FaDu (C) and Detroit 562 (D) cell lines. Cell lines were permeabilized and immunostained with TLR3-specific antibody (thick black line) or isotype-control antibody (filled grey area). Fluorescence tracings are representative of three independent experiments. SW620 and Detroit 562 cells were stained with 1 µg, SW480 with 1.5 µg and FaDu with 2 µg of TLR3 antibody and isotope control respectively.

    See Abreview

References for Anti-TLR3 antibody [40C1285] (ab13915)

This product has been referenced in:
  • Ni YH  et al. Distinct expression patterns of Toll-like receptor 7 in tumour cells and fibroblast-like cells in oral squamous cell carcinoma. Histopathology 67:730-9 (2015). IHC . Read more (PubMed: 25828894) »
  • Sistigu A  et al. Cancer cell-autonomous contribution of type I interferon signaling to the efficacy of chemotherapy. Nat Med 20:1301-9 (2014). Read more (PubMed: 25344738) »

See all 10 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Dec 01 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (Macrophages)
Specification Macrophages
Permeabilization Yes - Triton X 0.1% 5 min
Fixative Formaldehyde
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Submitted Jul 23 2014

Thank you for contacting us.

Unfortunately the epitope for these two antibodies has not been mapped, and therefore I am unable to provide the sequence they recognise.

However, ab13915 is currently in stock. If this antibody gave you...

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Thank you for contacting us. This antibody has not been tested in frozen sections or in cells, or in paraffin embedded tissue sections. Typically though, antibodies that work well in paraffin embedded sections also work in frozen tissues.
I hope th...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
I have read the details you have kindly provided and have following further suggestions;
- Try loading 30-40 ug of protein lysates.
-...

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Thank you for completing the survey.

I am glad to hear that the product worked fine at the end. Could you share the details of step you have altered for better results; it may help other scientists in community?

Many thanks for your c...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

The protocol seems fine to me however because antibodies needs optimization when they used with paraffin embedded sections so I have foll...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am at...

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Thank you for contacting us.

We do notsell blocking peptide for ab12121 so I am sorry we will not be able to provide this.

I have been waiting an answer from lab regarding ab13915 and ab45371; I will send you the info soon.

I...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab13915.

To check the status of the order please contact o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"