Anti-TLR3 antibody [40C1285.6] (Phycoerythrin) (ab45093)

Overview

  • Product nameAnti-TLR3 antibody [40C1285.6] (Phycoerythrin)
    See all TLR3 primary antibodies
  • Description
    Mouse monoclonal [40C1285.6] to TLR3 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This antibody was developed against KLH-conjugated synthetic peptide of human TLR3.

  • Positive control
    • Human intestine or Ramos.

Properties

Applications

Our Abpromise guarantee covers the use of ab45093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Flow Cyt
  • Application notesFACS:(Intracellular) Use 2 - 5µg for 106 cells.
    WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 104 kDa.

    This antibody has tested negative by western blot in mouse intestine cell lysate.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • Tissue specificityExpressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
    • Involvement in diseaseDefects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
    • Sequence similaritiesBelongs to the Toll-like receptor family.
      Contains 22 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 TIR domain.
    • Domainds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
    • Post-translational
      modifications
      Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
    • Cellular localizationEndoplasmic reticulum membrane. Endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD283 antibody
      • CD283 antigen antibody
      • IIAE2 antibody
      • TLR 3 antibody
      • Tlr3 antibody
      • TLR3_HUMAN antibody
      • Toll Like Receptor 3 antibody
      • Toll-like receptor 3 antibody
      see all

    Anti-TLR3 antibody [40C1285.6] (Phycoerythrin) images

    • All lanes : Anti-TLR3 antibody [40C1285.6] (Phycoerythrin) (ab45093) at 1 µg/ml

      Lane 1 : A459 cell lysate
      Lane 2 : Human skin tissue lysate
      Lane 3 : Human lung tissue lysate
      Lane 4 : Human dendritic cell lysate
      Lane 5 : Human macrophage cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      HRP-conjugated Goat anti-mouse IgG at 1/2000 dilution

      Performed under reducing conditions.

      Predicted band size : 104 kDa
      Observed band size : 104 kDa


      Exposure time : 10 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview

    • ab45093 staining TLR3 in Human A549 (lung epithelial) cell line by Flow Cytometry. The sample was incubated with the primary antibody (5μg/ml in 0.1% Saponin + 1% FCS in PBS) for 45 minutes at 4°C. 1 = isotype control, 2 = ab45093

       

      See Abreview

    • ab45093, at 3 ug/ 1X10^6 cells, staining Ramos cells by Intracellular flow cytometric analysis. Toll-Like-Receptor3 (open peak) and isotype negative control (shaded peak).

    References for Anti-TLR3 antibody [40C1285.6] (Phycoerythrin) (ab45093)

    This product has been referenced in:
    • Franzen-Röhl E  et al. Increased cell-mediated immune responses in patients with recurrent herpes simplex virus type 2 meningitis. Clin Vaccine Immunol 18:655-60 (2011). Flow Cyt . Read more (PubMed: 21325490) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gels)
    Sample Human Tissue lysate - whole (see below)
    Specification see below
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 01 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Fixation Paraformaldehyde
    Permeabilization Yes - 0,1% Saponin / PBS
    Sample Human Cell (A549 cells (lung epithelial cells))
    Specification A549 cells (lung epithelial cells)
    Gating Strategy no gate
    Preparation Cell harvesting/tissue preparation method: cell culture (commercially available cell line)
    Sample buffer: 0,1% Saponin /1% FCS/ PBS
    Username

    Abcam user community

    Verified customer

    Submitted Jul 23 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"