A peptide corresponding to 15 amino acids near the carboxy terminus of human TLR3 (Peptide available as ab125950).
Our Abpromise guarantee covers the use of ab62566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).Can be blocked with TLR3 peptide (ab125950).|
Paraffin-embedded mouse spleen tissue stained for TLR3 using ab62566 at 2 µg/ml in immunohistochemical analysis.
Paraffin-embedded human spleen tissue stained for TLR3 using ab62566 at 5 µg/ml in immunohistochemical analysis.