Overview

  • Product name
  • Description
    Rabbit polyclonal to TLR3
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A peptide corresponding to 15 amino acids near the carboxy terminus of human TLR3 (Peptide available as ab125950).

  • Positive control
    • WB: Daudi cell lysate. IHC-P: Human and mouse spleen. ICC/IF: EL4 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
ICC Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).Can be blocked with TLR3 peptide (ab125950).

Target

  • Function
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • Involvement in disease
    Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • Domain
    ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • Post-translational
    modifications
    Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • Cellular localization
    Endoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all

Anti-TLR3 antibody images

  • Paraffin-embedded mouse spleen tissue stained for TLR3 using ab62566 at 2 µg/ml in immunohistochemical analysis.

  • Lane 1 : Anti-TLR3 antibody (ab62566) at 1 µg/ml
    Lane 2 : Anti-TLR3 antibody (ab62566) at 2 µg/ml

    Lane 1 : Daudi cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 104 kDa
    Observed band size : 104 kDa
  • Paraffin-embedded human spleen tissue stained for TLR3 using ab62566 at 5 µg/ml in immunohistochemical analysis.

  • Immunofluorescence of TLR3 in El4 cells using ab62566 at 10 ug/ml.

  • ab62566, at 1µg/ml, staining TLR3 in EL4 cells by immunocytochemistry.

References for Anti-TLR3 antibody (ab62566)

This product has been referenced in:
  • Shan M  et al. TIR-Domain-Containing Adapter-Inducing Interferon-ß (TRIF) Is Essential for MPTP-Induced Dopaminergic Neuroprotection via Microglial Cell M1/M2 Modulation. Front Cell Neurosci 11:35 (2017). WB . Read more (PubMed: 28275337) »
  • Ballak DB  et al. TLR-3 is present in human adipocytes, but its signalling is not required for obesity-induced inflammation in adipose tissue in vivo. PLoS One 10:e0123152 (2015). WB . Read more (PubMed: 25867514) »

See all 7 Publications for this product

Product Wall

Application
Western blot
Sample
Cynomolgus Monkey Tissue lysate - whole (macaque retina)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
300 µg
Specification
macaque retina
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 05 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (Macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Specification
Macaque retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Nov 02 2016

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Nov 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Mouse Tissue sections (whole embryo sections)
Specification
whole embryo sections
Permeabilization
Yes - 0.1% triton x100 in TBS
Fixative
Formaldehyde
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Submitted Aug 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 4%
Antigen retrieval step
Heat mediated
Sample
Human Tissue sections (brain)
Specification
brain
Permeabilization
No
Fixative
Formaldehyde
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Submitted Jun 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Primary lung epithelial cell)
Loading amount
20 µg
Specification
Primary lung epithelial cell
Treatment
10 ng/ml Poly I poly C
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Non-reduced Denaturing (7,5% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Oct 16 2012

Muchas gracias pos facilitarnos estos detalles. Estudiando el protocolo, todo parece correcto, no tenemos ninguna sugerencia que hacer. Es posible que el lote de anticuerpo concreto que recibisteis no funcionara correctamente.

Lamento mucho l...

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Gracias por contactarnos. Lamento mucho que el cliente esté teniendo problemas con nuestros productos, y agradezco enormemente que nos haya mandado las imágenes y la información del protocolo.

Sin embargo, para tratar de ...

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Thank you for contacting us.
We do not publish this information for all antibodies. The antibodies should detect denatured and reduced form of protein, if information is not given on the datasheet. If these does detect native form then it will be ...

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