Overview

  • Product nameAnti-TLR3 antibody
    See all TLR3 primary antibodies
  • Description
    Rabbit polyclonal to TLR3
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A peptide corresponding to 15 amino acids near the carboxy terminus of human TLR3 (Peptide available as ab125950).

  • Positive control
    • Daudi cell lysate. EL4 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
ICC Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).Can be blocked with TLR3 peptide (ab125950).

Target

  • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityExpressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • Involvement in diseaseDefects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • Domainds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • Post-translational
    modifications
    Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • Cellular localizationEndoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all

Anti-TLR3 antibody images

  • Lane 1 : Anti-TLR3 antibody (ab62566) at 1 µg/ml
    Lane 2 : Anti-TLR3 antibody (ab62566) at 2 µg/ml

    Lane 1 : Daudi cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 104 kDa
    Observed band size : 104 kDa
  • Immunohistochemistry of TLR3 in human spleen tissue with TLR3 antibody at 5 µg/ml.

  • Immunofluorescence of TLR3 in El4 cells using ab62566 at 10 ug/ml.

  • ab62566, at 1µg/ml, staining TLR3 in EL4 cells by immunocytochemistry.

References for Anti-TLR3 antibody (ab62566)

This product has been referenced in:
  • Ballak DB  et al. TLR-3 is present in human adipocytes, but its signalling is not required for obesity-induced inflammation in adipose tissue in vivo. PLoS One 10:e0123152 (2015). WB . Read more (PubMed: 25867514) »
  • Tsai SY  et al. Regulation of TLR3 Activation by S100A9. J Immunol 195:4426-37 (2015). Read more (PubMed: 26385519) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Cynomolgus Monkey Tissue lysate - whole (macaque retina)
Gel Running Conditions Reduced Denaturing (4-15%)
Loading amount 300 µg
Specification macaque retina
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 05 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cynomolgus Monkey Tissue sections (Macaque retina)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization No
Specification Macaque retina
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Nov 02 2016

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Nov 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Mouse Tissue sections (whole embryo sections)
Specification whole embryo sections
Permeabilization Yes - 0.1% triton x100 in TBS
Fixative Formaldehyde
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Submitted Aug 29 2013

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 4%
Antigen retrieval step Heat mediated
Sample Human Tissue sections (brain)
Specification brain
Permeabilization No
Fixative Formaldehyde
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Submitted Jun 10 2013

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Application Western blot
Sample Human Cell lysate - whole cell (Primary lung epithelial cell)
Loading amount 20 µg
Specification Primary lung epithelial cell
Treatment 10 ng/ml Poly I poly C
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Mar 18 2013

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Application Western blot
Sample Rat Cell lysate - whole cell (Liver)
Loading amount 20 µg
Specification Liver
Gel Running Conditions Non-reduced Denaturing (7,5% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Oct 16 2012

Muchas gracias pos facilitarnos estos detalles. Estudiando el protocolo, todo parece correcto, no tenemos ninguna sugerencia que hacer. Es posible que el lote de anticuerpo concreto que recibisteis no funcionara correctamente.

Lamento mucho l...

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Gracias por contactarnos. Lamento mucho que el cliente esté teniendo problemas con nuestros productos, y agradezco enormemente que nos haya mandado las imágenes y la información del protocolo.

Sin embargo, para tratar de ...

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Thank you for contacting us.
We do not publish this information for all antibodies. The antibodies should detect denatured and reduced form of protein, if information is not given on the datasheet. If these does detect native form then it will be ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"