Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human TLR3.
(Peptide available as ab93793.)
Our Abpromise guarantee covers the use of ab83338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC image of TLR3 staining in mouse kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83338, 5µg/ml, for 15 mins at room temperature. A goat anti rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab83338 has not yet been referenced specifically in any publications.