Overview

  • Product nameAnti-TLR3 antibody
    See all TLR3 primary antibodies
  • Description
    Rabbit polyclonal to TLR3
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human TLR3.

    (Peptide available as ab93793.)

  • Positive control
    • This antibody gave a positive signal in the following mouse lysates: Mouse lung tissue, NIH3T3 whole cell, A20 whole cell. IHC-P: FFPE mouse kidney tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab83338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 103 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • Tissue specificityExpressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
    • Involvement in diseaseDefects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
    • Sequence similaritiesBelongs to the Toll-like receptor family.
      Contains 22 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 TIR domain.
    • Domainds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
    • Post-translational
      modifications
      Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
    • Cellular localizationEndoplasmic reticulum membrane. Endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD283 antibody
      • CD283 antigen antibody
      • IIAE2 antibody
      • TLR 3 antibody
      • Tlr3 antibody
      • TLR3_HUMAN antibody
      • Toll Like Receptor 3 antibody
      • Toll-like receptor 3 antibody
      see all

    Anti-TLR3 antibody images

    • IHC image of TLR3 staining in mouse kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83338, 5µg/ml, for 15 mins at room temperature. A goat anti rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-TLR3 antibody (ab83338) at 1 µg/ml

      Lane 1 : Mouse lung normal tissue lysate - total protein (ab29297)
      Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 3 : A20 whole cell lysate (ab7180)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 103 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 20 minutes
      The band observed at 100 kDa could potentially be a cleaved form of Mouse Toll-like receptor 3 precursor. TLR3 contains a 25 amino acid signal peptide, which when removed gives an expected molecular weight of 100 kDa.

    References for Anti-TLR3 antibody (ab83338)

    ab83338 has not yet been referenced specifically in any publications.

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    This antibody can be conjugated, however the buffers components 1% BSA and 0.02% sodium azide can interfere with the conjugation process. The concentrations of sodium azide is very-very less so it should not be a probl...

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