Overview

  • Product name
    Anti-TLR3 antibody [TLR3.7]
    See all TLR3 primary antibodies
  • Description
    Mouse monoclonal [TLR3.7] to TLR3
  • Specificity
    Ab12085 recognises TLR3.
  • Tested applications
    Suitable for: Inhibition Assay, Functional Studies, IHC-P, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Dog, Human, Pig
  • Immunogen

    Human Flag-tagged TLR3 stably expressed by Ba/F3 cells.

  • Positive control
    • Placenta and pancreas. Monocyte-derived immature dendritic cells (see paper by Matsumoto et al,2003). Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab12085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration. Use ligand poly(I):poly(C).
Functional Studies Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 22023039
IP Use at an assay dependent concentration. See paper by Matsumoto et al, 2003.
Flow Cyt 1/10. See paper by Matsumoto et al, 2003.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 19656899
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • Tissue specificity
      Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
    • Involvement in disease
      Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
    • Sequence similarities
      Belongs to the Toll-like receptor family.
      Contains 22 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 TIR domain.
    • Domain
      ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
    • Post-translational
      modifications
      Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
    • Cellular localization
      Endoplasmic reticulum membrane. Endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD283 antibody
      • CD283 antigen antibody
      • IIAE2 antibody
      • TLR 3 antibody
      • Tlr3 antibody
      • TLR3_HUMAN antibody
      • Toll Like Receptor 3 antibody
      • Toll-like receptor 3 antibody
      see all

    Images

    • Flow cytometric detection of human TLR3 on THP-1 cells. Red line represents the isotype control and the black line represents ab12085 at 10 μg/ml.

    References

    This product has been referenced in:
    • Hwang SH  et al. Characteristics of Human Turbinate-Derived Mesenchymal Stem Cells Are Not Affected by Allergic Condition of Donor. PLoS One 10:e0138041 (2015). Read more (PubMed: 26376485) »
    • Yuan MM  et al. TLR3 expression correlates with apoptosis, proliferation and angiogenesis in hepatocellular carcinoma and predicts prognosis. BMC Cancer 15:245 (2015). IHC . Read more (PubMed: 25884709) »

    See all 9 Publications for this product

    Customer reviews and Q&As

    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - Triton X
    Sample
    Pig Cell (PBMC)
    Specification
    PBMC
    Gating Strategy
    lymphocytes
    Preparation
    Cell harvesting/tissue preparation method: isolation from blood
    Sample buffer: PBS
    Username

    Korneel Grauwet

    Verified customer

    Submitted Feb 06 2014

    I heard back from our lab that the most recent lot of ab12085 had an endotoxin level of 0.02EU/ml. The ab17264 has not been tested for endotoxin.



    I can confirm that IgG1 has a mass of ca 150kDa.

    http://www.abcam.com/index.html?pageconfig=resource&rid=11258&pid=10996

    This should not change very much between different mouse IgG1 (unconjugated) antibodies.
    Read More

    Thank you for contacting us.


    The biological activity of the antibody has been described in rthe following reference from datasheet:

    Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 tha...

    Read More

    Thank you for contacting us.


    ab12085 can be used to inhibit the function of TLR3. The following publication describe the use of this clone in an inhibitory assay:

    Matsumoto, M et al; Establishment of a monoclonal antibody agai...

    Read More

    Thank you for contacting us regarding ab65355, cAMP Direct Immunoassay kit. As requested I have created a credit note for the original purchase. Your credit note ID is . I am sorry that this antibody did not perform as stated on the datashee...

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    Thank you for contacting us. Unfortunately Anti-TLR3 antibody [TLR3.7] (ab12085) has not been tested for neutralising activity in rat. More information on the antibodies functional activity can be found from the following reference: Matsumoto M...

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    Thank you for contacting us. The following reference describes the development of the TLR3.7 clone and its use in an inhibition assay: Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blo...

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    以外と製造元の返事が早かったです。 この回答は電話で申し上げにくいかと思いますので、メールで送った方がよいではないかと思います。 ***製造元よりの情報*** 中和(inhibition)の詳細は下記の文献に載ってありますので、ご参考いただけますようお願いいたします。 1) Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blo...

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    Thank you for contacting us for technical support regarding ab12085. This antibody has not been tested against other species than human and I would recommend running in parallel with your samples the positive controls of human placenta or pancreas ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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