Overview

  • Product nameAnti-TLR3 antibody [TLR3.7]
    See all TLR3 primary antibodies
  • Description
    Mouse monoclonal [TLR3.7] to TLR3
  • SpecificityAb12085 recognises TLR3.
  • Tested applicationsSuitable for: Inhibition Assay, Functional Studies, IHC-P, WB, IP, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Dog, Human, Pig
  • Immunogen

    Human Flag-tagged TLR3 stably expressed by Ba/F3 cells.

  • Positive control
    • Placenta and pancreas. Monocyte-derived immature dendritic cells (see paper by Matsumoto et al,2003). Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab12085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration. Use ligand poly(I):poly(C).
Functional Studies Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/50. Predicted molecular weight: 103.8 kDa.
IP Use at an assay dependent concentration. See paper by Matsumoto et al, 2003.
Flow Cyt 1/10. See paper by Matsumoto et al, 2003.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionKey component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityExpressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • Involvement in diseaseDefects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • Domainds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • Post-translational
    modifications
    Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • Cellular localizationEndoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all

References for Anti-TLR3 antibody [TLR3.7] (ab12085)

This product has been referenced in:
  • Hwang SH  et al. Toll like receptor 3 & 4 responses of human turbinate derived mesenchymal stem cells: stimulation by double stranded RNA and lipopolysaccharide. PLoS One 9:e101558 (2014). Flow Cyt ; Human . Read more (PubMed: 25004159) »
  • Hashad DI  et al. Toll like receptor 3 expression as a novel predictor of response to treatment in chronic hepatitis C virus patients. Scand J Clin Lab Invest 71:641-6 (2011). IHC-P ; Human . Read more (PubMed: 22023039) »

See all 7 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - Triton X
Sample Pig Cell (PBMC)
Specification PBMC
Gating Strategy lymphocytes
Preparation Cell harvesting/tissue preparation method: isolation from blood
Sample buffer: PBS
Username

Korneel Grauwet

Verified customer

Submitted Feb 06 2014

I heard back from our lab that the most recent lot of ab12085 had an endotoxin level of 0.02EU/ml. The ab17264 has not been tested for endotoxin.



I can confirm that IgG1 has a mass of ca 150kDa.

http://www.abcam.com/index.html?pageconfig=resource&rid=11258&pid=10996

This should not change very much between different mouse IgG1 (unconjugated) antibodies.
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Thank you for contacting us.


The biological activity of the antibody has been described in rthe following reference from datasheet:

Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 tha...

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Thank you for contacting us.


ab12085 can be used to inhibit the function of TLR3. The following publication describe the use of this clone in an inhibitory assay:

Matsumoto, M et al; Establishment of a monoclonal antibody agai...

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Thank you for contacting us regarding ab65355, cAMP Direct Immunoassay kit. As requested I have created a credit note for the original purchase. Your credit note ID is . I am sorry that this antibody did not perform as stated on the datashee...

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Thank you for that additional information.

I would suggest two small changes to your protocol which may help. First, try using another blocking reagent in place of milk. A casein based blocking buffer such as our Mitosciences 10X Blocking Buff...

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I am glad to help you to resolve this issue.

Would you be able to send me the protocol details for your experiment? I will review these and determine if changes in your protocol may help. This product is also covered by our Abpromise guarante...

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Thank you for contacting us. Unfortunately Anti-TLR3 antibody [TLR3.7] (ab12085) has not been tested for neutralising activity in rat. More information on the antibodies functional activity can be found from the following reference: Matsumoto M...

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Thank you for contacting us. The following reference describes the development of the TLR3.7 clone and its use in an inhibition assay: Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blo...

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