Anti-TLR4 antibody [76B357.1] (FITC) (ab45126)

Overview

  • Product nameAnti-TLR4 antibody [76B357.1] (FITC)
    See all TLR4 primary antibodies
  • Description
    Mouse monoclonal [76B357.1] to TLR4 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Sheep, Horse, Cow, Cat, Pig, Chimpanzee, Baboon, Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to Human TLR4 aa 100-200.
    Database link: O00206

  • Positive control
    • Ramos or THP1

Properties

Applications

Our Abpromise guarantee covers the use of ab45126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2-5µg for 106 cells. ab18427-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • DomainThe TIR domain mediates interaction with NOX4.
  • Post-translational
    modifications
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody [76B357.1] (FITC) images

  • Immunofluorescence analysis of 16HBE14o- cells, staining TLR4 (red) with ab45126. Cells were fixed with paraformaldehyde for 10 min and permeabilized with 0.2% Triton X-100 in PBS for 10 min before incubating with primary antibody for 1 hour.
  • FACS analysis of cell surface TLR4 in human monocyte-derived dendritic cells using ab45126 at 1/200 (2.5 µg). Black and green open histograms represent unstained cells and isotype control (anti-human CD14 FITC) respectively. Blue open histogram is ab45126 anti-TLR4 stained dendritic cells. Data courtesy Anna Manlapat, National Institutes of Health

References for Anti-TLR4 antibody [76B357.1] (FITC) (ab45126)

This product has been referenced in:
  • Ambarus CA  et al. Soluble Immune Complexes Shift the TLR-Induced Cytokine Production of Distinct Polarized Human Macrophage Subsets towards IL-10. PLoS One 7:e35994 (2012). Flow Cyt ; Human . Read more (PubMed: 22563430) »
  • Gries M  et al. Response of Toll-like receptors in experimental Guillain-Barré syndrome: a kinetic analysis. Neurosci Lett 518:154-60 (2012). Read more (PubMed: 22579825) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Sheep Cell (Macrophage)
Permeabilization No
Gating Strategy SSC FSC
Specification Macrophage
Fixation Paraformaldehyde
Username

Mr. Jifan Yang

Verified customer

Submitted Feb 23 2016


From the antibodies you have been looking at, I would suggest ab45126 would be most suitable depending on the application you hope to use the antibody in. The antibody ab95563 is directed against the mouse Toll-like receptor 4 (TLR4)/MD-2. The hom...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Application Western blot
Sample Human Cell lysate - whole cell (Colon cancer (tissue lysate), U2OS (cell lysate) a)
Loading amount 20 µg
Specification Colon cancer (tissue lysate), U2OS (cell lysate) a
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"