Overview

  • Product name
  • Description
    Rabbit polyclonal to TLR4
  • Specificity
    TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate.
  • Tested applications
    Suitable for: ICC/IF, Electron Microscopy, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to TLR4 aa 39-56.
    Sequence:

    CMDQKLSKVPDDIPSSTK

  • Positive control
    • WB: Partial recombinant mouse TLR4 (extracellular portion plus His-tag), RAW cell lysate, Daudi cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab13867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 16959900
Electron Microscopy Use at an assay dependent concentration. PubMed: 24489676
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 96 kDa). Recommended lysis buffer: 10 mM Tris, pH8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi (tetrasodium Pyrophosphate)(supplemented with protease inhibitor cocktail).

Target

  • Function
    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificity
    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in disease
    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Domain
    The TIR domain mediates interaction with NOX4.
  • Post-translational
    modifications
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody images

  • Analysis of TLR4 in mouse kidney tissue using ab13867 at 5µg/ml.



  • Predicted band size : 96 kDa

    Western blot analysis of TLR4 using ab13867 at 2 ug/ml on A) 0.1 ug/lane partial recombinant mouse TLR4 protein and B) RAW cell lysate and C) Daudi cell lysate. Western blot analysis of TLR4 using ab13867 at 2 ug/ml on A) 0.1 ug/lane partial recombinant mouse TLR4 protein and B) RAW cell lysate (ab7187) and C) Daudi cell lysate (ab3951).

References for Anti-TLR4 antibody (ab13867)

This product has been referenced in:
  • Chen L  et al. Relaxin abrogates renal interstitial fibrosis by regulating macrophage polarization via inhibition of Toll-like receptor 4 signaling. Oncotarget 8:21044-21053 (2017). WB ; Mouse . Read more (PubMed: 28416741) »
  • Zhang Q  et al. Toll-like receptor 4 contributes to acute kidney injury after cardiopulmonary resuscitation in mice. Mol Med Rep 14:2983-90 (2016). Mouse . Read more (PubMed: 27510583) »

See all 9 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Mouse Tissue sections (frozen sections from mouse embryo)
Specification
frozen sections from mouse embryo
Permeabilization
Yes - triton-x 0.1% in TBS
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 03 2014

Thank you for calling Abcam.

I have sent you a vial of ab29545, HeLa cell lysate to use as a positive control.

Please let me know if there is anything else I can help you with, or if continue to experience any issue with our antibod...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lungs)
Loading amount
30 µg
Specification
Lungs
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 26 2009

I'm sorry to hear you are experiencing problems with ab13867. The problems you are experiencing could be due to a number of reasons, such as low levels of TLR4 in your samples (due to the transfection procedure or the extraction procedure) and there...

Read More

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