Overview

  • Product nameAnti-TLR4 antibody
    See all TLR4 primary antibodies
  • Description
    Rabbit polyclonal to TLR4
  • SpecificityTLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate.
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TLR4 aa 420-435.
    Sequence:

    GLEQLEHLDFQHSNLK


    Database link: O00206

  • Positive control
    • Recombinant mouse TLR4 protein, normal and tumor prostate sections, Ramos cells and 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab47093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. PubMed: 21540291
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/50.
IHC-P 1/50.

Target

  • FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • DomainThe TIR domain mediates interaction with NOX4.
  • Post-translational
    modifications
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody images

  • ICC analysis of TLR4 in formalin-fixed Ramos (a) and 293 (b) cells using ab47093 at 1:10 dilution. Data courtesy of Dr. Regina Gandour-Edwards.
  • Immunohistochemistry analysis of TLR4 in formalin-fixed, paraffin-embedded normal (a) and tumor (b) prostate sections using ab47093 at 1:50 dilution. Data courtesy of Dr. Regina Gandour-Edwards.
  • Flow Cytometry: Cell surface analysis using ab47093. Mouse macrophage cells were probed using 1 ug of TLR4 antibody (red) and 1 ug of isotype control (PE-conjugated secondary) antibody (green).

  • WB analysis of TLR4 using ab47093 at 2 ug/ml on partial recombinant mouse TLR4 protein.

     

References for Anti-TLR4 antibody (ab47093)

This product has been referenced in:
  • Tang YL  et al. TLR4/NF-?B signaling contributes to chronic unpredictable mild stress-induced atherosclerosis in ApoE-/- mice. PLoS One 10:e0123685 (2015). WB ; Mouse . Read more (PubMed: 25860573) »
  • Lucas AR  et al. Myxomavirus anti-inflammatory chemokine binding protein reduces the increased plaque growth induced by chronic Porphyromonas gingivalis oral infection after balloon angioplasty aortic injury in mice. PLoS One 9:e111353 (2014). IHC ; Mouse . Read more (PubMed: 25354050) »

See all 12 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Hepatocarcinoma (HCC))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC
Permeabilization No
Specification Hepatocarcinoma (HCC)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 22 2015

Thank you very much for your reply.

We would very much appreciate it if you would give us feedback on how ab47903 works with the Benchmark stainer. We will fully guarantee this antibody to work on paraffin-embedded tissue, so if you do have a...

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Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from these antibodies.

The details your customer has kindly provi...

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Thank you very much for contacting us with your enquiry.

I've checked with the lab, and this TLR4 antibody is tested using a benchtop protocol, not in an auto-stainer. It's possible that it will work with the Ventana Benchmark but we have not...

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Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product qu...

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Vielen Dank für Ihre Email.

Ich denke, ein alternativer Antikörper ist vielleicht die bessere Wahl.

Ich habe Ihnen jeweils einen Antikörper für TLR2 und TLR4 herausgesucht:

http://www.abcam.com/index.h...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the timethe customer has spent on these experiments and would be plea...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for your reply. As an alternative positive control, you could try human colon whole cell lysate: ab44213. This is unfortunately the smallest size we currently sell for lysates.

DISCOUNT CODE: ***
Expiration date: November 12th, ...

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Thank you for your reply.

For positive controls, I would recommend the human colon slides: ab4327 and small intestine whole cell lysate from either human (ab29276) or mouse (ab7939). I hope this helps, please let me know if you need any addi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"