• Product nameAnti-TLR4 antibody
    See all TLR4 primary antibodies
  • Description
    Rabbit polyclonal to TLR4
  • SpecificityTLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control mouse intestine tissue lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse TLR4.

    (Peptide available as ab93304.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: NIH3T3; EL4.


Associated products


Our Abpromise guarantee covers the use of ab83444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100-125 kDa (predicted molecular weight: 95 kDa).

TLR4 has a predicted molecular weight of 95 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data).


  • FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • DomainThe TIR domain mediates interaction with NOX4.
  • Post-translational
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody images

  • All lanes : Anti-TLR4 antibody (ab83444) at 5 µg/ml

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : Mouse small intestines tissue lysate

    Lysates/proteins at 20 µg per lane.

    IR-goat anti-rabbit IgG (green) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 95 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)

    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab83444 (rabbit polyclonal to TLR4; 5 ug per mL) and ab8245 (mouse monoclonal to GAPDH, loading control; 1/20 000), overnight at 4°C. Antibody binding was detected using infrared (IR) labelled goat anti-rabbit (green) and IR-labelled goat anti-mouse (red) at 1/20 000 dilutions for 1 hour at room temperature before imaging.

  • All lanes : Anti-TLR4 antibody (ab83444) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : EL4 whole cell lysate (ab7183)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 95 kDa
    Additional bands at : 120 kDa (possible glycosylated form),125 kDa (possible glycosylated form).

    Exposure time : 20 minutes

    TLR4 has a predicted molecular weight of 95 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data) explaining the bands observed at 120 and 125 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References for Anti-TLR4 antibody (ab83444)

ab83444 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. I apologize for the delay. The catalogue number of blocking peptide is ab116167. The price is £110 for 100 ug size. I hope this information is helpful to you. Please do not hesitate to contact us if yo...

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Thank you for contacting us. The positive controls for ab95023 are cell lysates of A-431, U2OS, U251 MG, Hela cell etc. Please click the following to see the positive control tissue lysates; http://www.proteinatlas.org/ENSG00000168685 ...

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