Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse TLR4.
(Peptide available as ab93304.)
Our Abpromise guarantee covers the use of ab83444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100-125 kDa (predicted molecular weight: 95 kDa).
TLR4 has a predicted molecular weight of 95 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data).
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab83444 (rabbit polyclonal to TLR4; 5 ug per mL) and ab8245 (mouse monoclonal to GAPDH, loading control; 1/20 000), overnight at 4°C. Antibody binding was detected using infrared (IR) labelled goat anti-rabbit (green) and IR-labelled goat anti-mouse (red) at 1/20 000 dilutions for 1 hour at room temperature before imaging.
TLR4 has a predicted molecular weight of 95 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data) explaining the bands observed at 120 and 125 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab83444 has not yet been referenced specifically in any publications.