Anti-TLR4 antibody [HTA125] (ab8376)
Key features and details
- Mouse monoclonal [HTA125] to TLR4
- Suitable for: IP, Flow Cyt
- Reacts with: Rat, Guinea pig, Human, Pig, Rhesus monkey
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-TLR4 antibody [HTA125]
See all TLR4 primary antibodies -
Description
Mouse monoclonal [HTA125] to TLR4 -
Host species
Mouse -
Specificity
This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4 has been demonstrated to act as alpha receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. We do not guarantee WB since we receive variable results from our customers and cannot make this antibody work in our hands in WB in denaturing and reducing conditions. The immunogen is a full-length immunogen so we suspect that to get the product to work in WB, non-reducing or native WB could be a better option. THP1 monocytes or macrophage are good positive controls and HeLa/Hek293 should be suitable negative/low expression controls. Membrane preparation may be better that whole cell as expression levels may not be that high in some samples. We welcome any further feedback in WB by participating to our AbTrial program. -
Tested applications
Suitable for: IP, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Guinea pig, Human, Pig, Rhesus monkey -
Immunogen
Tissue, cells or virus corresponding to TLR4. Ba/F3 cell line expressing TLR4
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HTA125 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
1/10 - 1/25.
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Method sheets are available on request.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Notes |
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IP
Use at an assay dependent concentration. |
Flow Cyt
1/10 - 1/25. Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Method sheets are available on request.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Function
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
Tissue specificity
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
Involvement in disease
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Domain
The TIR domain mediates interaction with NOX4. -
Post-translational
modificationsN-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7099 Human
- Entrez Gene: 399541 Pig
- Entrez Gene: 29260 Rat
- Omim: 603030 Human
- SwissProt: O00206 Human
- SwissProt: Q68Y56 Pig
- SwissProt: Q9QX05 Rat
- Unigene: 174312 Human
see all -
Alternative names
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab8376 has been referenced in 30 publications.
- Wardani G et al. Antioxidative Stress and Anti-Inflammatory Activity of Fucoidan Nanoparticles against Nephropathy of Streptozotocin-Induced Diabetes in Rats. Evid Based Complement Alternat Med 2022:3405871 (2022). PubMed: 35685736
- Wang X et al. Danshen (Salvia miltiorrhiza) restricts MD2/TLR4-MyD88 complex formation and signalling in acute myocardial infarction-induced heart failure. J Cell Mol Med 24:10677-10692 (2020). PubMed: 32757377
- Shi L et al. Chitosan oligosaccharide-mediated attenuation of LPS-induced inflammation in IPEC-J2 cells is related to the TLR4/NF-?B signaling pathway. Carbohydr Polym 219:269-279 (2019). PubMed: 31151525
- Kim SY et al. Isorhamnetin alleviates lipopolysaccharide-induced inflammatory responses in BV2 microglia by inactivating NF-?B, blocking the TLR4 pathway and reducing ROS generation. Int J Mol Med 43:682-692 (2019). PubMed: 30483725
- Cebatariuniene A et al. Extracellular Vesicles Suppress Basal and Lipopolysaccharide-Induced NF?B Activity in Human Periodontal Ligament Stem Cells. Stem Cells Dev N/A:N/A (2019). PubMed: 31017040