Overview

  • Product nameAnti-TLR4 antibody [HTA125]
    See all TLR4 primary antibodies
  • Description
    Mouse monoclonal [HTA125] to TLR4
  • SpecificityThis antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4 has been demonstrated to act as alpha receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate.
  • Tested applicationsSuitable for: Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Rat, Guinea pig, Human, Pig, Rhesus monkey
  • Immunogen

    Ba/F3 cell line expressing TLR4

Properties

Applications

Our Abpromise guarantee covers the use of ab8376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/25. Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Method sheets are available on request.



ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • DomainThe TIR domain mediates interaction with NOX4.
  • Post-translational
    modifications
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody [HTA125] images

  • Staining of human peripheral blood monocytes with ab8376.

References for Anti-TLR4 antibody [HTA125] (ab8376)

This product has been referenced in:
  • d'Adhemar CJ  et al. The MyD88+ Phenotype Is an Adverse Prognostic Factor in Epithelial Ovarian Cancer. PLoS One 9:e100816 (2014). WB ; Human . Read more (PubMed: 24977712) »
  • Sanchez-Pernaute O  et al. Citrullination enhances the pro-inflammatory response to fibrin in rheumatoid arthritis synovial fibroblasts. Ann Rheum Dis : (2012). Read more (PubMed: 23234648) »

See all 12 Publications for this product

Product Wall

Thank you for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appr...

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Thank you for your email.

I have checked the images and based on the images and protocol, I have following suggestions;

- Use a different HRP conjugated antibody. DAKO ChemMate EnVision Detection Kit as per DAKO website is optimized f...

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Thank you very much sending details.

I am surprised to hear that antibody failed in 2 different occasions as we haven't received any complaint for these lots so I think protocol might need further optimizations. Could you provide image of stai...

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Thank you for completing the survey on Abcam website.

I am sorry to hear that the antibody did not work with porcine sample. I have attached a questionnaire, to follow up for the complaint. Could you complete this and send it to me asap. I wil...

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Thank you for your email.

The order number I have created is 1107878; you will be receiving this antibody shortly.

TLR4 is highly expressed in porcine kidney so the antibody should have worked. Please let me know if you experience any...

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I am sorry, I forgot to ask the order number of your last purchase. Could you send the purchase order number to us so that we can send the new vial at same address?

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DISCOUNT CODE: XXXXXXXXXXX (for ab8376)
DISCOUNT CODE: XXXXXXXXXXX (for ab11354)

Expiration date: 24-09-2012

I am very pleased to hear you would like to use ab8376 (WB) and ab11354(IHC-P) inPorcine and send us an image of you...

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Thank you for your reply. I'm afraid all the information we have is from those references and therefore we do not have any more data looking at the blocking ability of the antibody. We welcome all feedback on our antibodies via our Abreviews system ...

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I can confirm that the blocking activity of this clone has been established in a number of studies, for your convenience I have listed some useful articles below: 1: Infect Immun. 2001 Apr;69(4):2045-53. Synergistic effect of muramyldipeptide wit...

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Unfortunately I do not believe that the epitope that this antibody recognises has been fully characterised and I have been unable to locate any further information relating to the terminus recognised. I am sorry that I cannot be of additional assistanc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"