Anti-TLR4 antibody [HTA125] (Biotin) (ab17232)


  • Product nameAnti-TLR4 antibody [HTA125] (Biotin)
    See all TLR4 primary antibodies
  • Description
    Mouse monoclonal [HTA125] to TLR4 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus Monkey, Rhesus monkey
  • Immunogen

    TLR4 expressing Ba/F3 cells.

  • Positive control
    • Human peripheral blood monocytes
  • General notes

    Ab17232 reacts preferentially, especially in flow cytometry, with human TLR4 associated with MD-2. It is a LR4 function blocking antibody that is useful for studies on the role of TR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells.



Our Abpromise guarantee covers the use of ab17232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50. ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/50.


  • FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • DomainThe TIR domain mediates interaction with NOX4.
  • Post-translational
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

References for Anti-TLR4 antibody [HTA125] (Biotin) (ab17232)

ab17232 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. Unfortunately I have not been able to obtain a specific protocol for this antiserum. However, we do have an excellent IHC-Fr protocol located at the following link:

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Thank you for your enquiry. Yes, ab17232 has been tested for application in IHC-frozen sections and tested for cross-reactivity with Human, Cynomolgus Monkey, Marmoset Monkey and Rhesus monkey. I would suggest a dilution range of 1:20-1:200, and optimi...

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