Ba/F3 cell line expressing TLR4.
Our Abpromise guarantee covers the use of ab30667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.
Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood.
|IP||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration. PubMed: 17767165|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17916800|
ICC/IF image of ab30667 stained rat adrenal medulla PC12 cells. The cells were 4% PFA and 100% Methanol fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30667, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).