Anti-TLR4 antibody [HTA125] (Phycoerythrin) (ab11227)

Overview

  • Product nameAnti-TLR4 antibody [HTA125] (Phycoerythrin)
    See all TLR4 primary antibodies
  • Description
    Mouse monoclonal [HTA125] to TLR4 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab11227 recognises the human Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Rhesus monkey
  • Immunogen

    Ba/F3 cell line expressing human TLR4.

  • Positive control
    • Human monocytes and macrophages/whole blood.
  • General notes

    This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

    For Flow Cytometry: Use 10 µl of undiluted antibody to label 106 cells or 100 µl whole blood.

Properties

Applications

Our Abpromise guarantee covers the use of ab11227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use neat.

    TLR4 expression on U937 cells can be upregulated following stimulation with e.g. LPS or IFN gamma.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
    • Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
    • Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    • Sequence similaritiesBelongs to the Toll-like receptor family.
      Contains 18 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 TIR domain.
    • DomainThe TIR domain mediates interaction with NOX4.
    • Post-translational
      modifications
      N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARMD10 antibody
      • CD284 antibody
      • CD284 antigen antibody
      • Homolog of Drosophila toll antibody
      • hToll antibody
      • TLR 4 antibody
      • TLR4 antibody
      • TLR4_HUMAN antibody
      • TOLL antibody
      • Toll like receptor 4 antibody
      • Toll-like receptor 4 antibody
      see all

    Anti-TLR4 antibody [HTA125] (Phycoerythrin) images

    • Staining of U937 cells with ab11227.
    • Ab11227 staining on human peripheral blood monocytes.

    References for Anti-TLR4 antibody [HTA125] (Phycoerythrin) (ab11227)

    ab11227 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your email. We are unable to measure the concentration of our PE conjugates, so I cannot give you precise details. However, the concentration of the antibody will be approximately 0.1mg/ml. I hope this information helps, please do n...

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    Thank you for your enquiry. Expression of TLR4 on normal resting monocytes is low and for this reason ab11227 is routinely QC'd on the U937 cell line which demonstrates a reasonable level of expression. I can attach an image here so I will send ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"