TLR4 overexpression 293T lysate (whole cell) (ab94063)

Overview

  • Product nameTLR4 overexpression 293T lysate (whole cell)
  • Description
    TLR4 overexpression lysate
  • General notesab94063 is a 293T cell transfected lysate in which Human TLR4 has been transiently over-expressed using a pCMV-TLR4 plasmid. The lysate is provided in 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundDisease: Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Domain: The TIR domain mediates interaction with NOX4. Function: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. PTM: N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. Similarity: Belongs to the Toll-like receptor family. Contains 18 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 TIR domain. Tissue specificity: Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

TLR4 overexpression 293T lysate (whole cell) images

  • ab94063 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-TLR4 antibody (ab89455) at 1/500 dilution

    Lane 1 : TLR4 293T Transfected Lysate - Positive Control (ab794063)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    non-Abcam, Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for TLR4 overexpression 293T lysate (whole cell) (ab94063)

ab94063 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"