Anti-TLR4 antibody (ab218987)
Key features and details
- Rabbit polyclonal to TLR4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TLR4 antibody
See all TLR4 primary antibodies -
Description
Rabbit polyclonal to TLR4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Macaque monkey -
Immunogen
Synthetic peptide within Human TLR4 aa 650-750 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O00206 -
Positive control
- Human prostate tissue; Human intestine tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 95 kDa.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 95 kDa. |
Target
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Function
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
Tissue specificity
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
Involvement in disease
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Domain
The TIR domain mediates interaction with NOX4. -
Post-translational
modificationsN-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281536 Cow
- Entrez Gene: 7099 Human
- Omim: 603030 Human
- SwissProt: Q9GL65 Cow
- SwissProt: Q9MYW3 Horse
- SwissProt: O00206 Human
- Unigene: 174312 Human
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Alternative names
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue, labeling TLR4 with ab218987 at 5 μg/ml.
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All lanes : Anti-TLR4 antibody (ab218987) at 0.1 µg/ml
Lane 1 : Human intestine lysate
Lane 2 : Human intestine lysate with Immunizing peptide
Secondary
All lanes : Goat anti-Rabbit Ig HRP
Predicted band size: 95 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab218987 has not yet been referenced specifically in any publications.