Overview

  • Product name
    Anti-TLR4/MD2 Complex antibody [MTS510]
    See all TLR4/MD2 Complex primary antibodies
  • Description
    Rat monoclonal [MTS510] to TLR4/MD2 Complex
  • Specificity
    The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Toll-like receptor 4 (TLR4)/MD-2 complex of Mouse origin

  • Positive control
    • Thioglycolate-induced mouse peritoneal exudate cells (PECs)

Properties

Applications

Our Abpromise guarantee covers the use of ab95562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 105-8 cells. Staining the cell sample in a final volume of 100 µL is recommended.



ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificity
    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in disease
    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Domain
    The TIR domain mediates interaction with NOX4.
  • Post-translational
    modifications
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • ESOP 1 antibody
    • ESOP1 antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • LY 96 antibody
    • LY96 antibody
    • Lymphocyte antigen 96 antibody
    • md 2 antibody
    • MD 2 protein antibody
    • MD2 protein antibody
    • Myeloid differentiation protein 2 antibody
    • Protein MD 2 antibody
    • Protein MD2 antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

References

This product has been referenced in:
  • Chabot S  et al. TLRs regulate the gatekeeping functions of the intestinal follicle-associated epithelium. J Immunol 176:4275-83 (2006). IHC-Fr . Read more (PubMed: 16547265) »
  • Mortaz E  et al. Acetylsalicylic acid-induced release of HSP70 from mast cells results in cell activation through TLR pathway. Exp Hematol 34:8-18 (2006). Read more (PubMed: 16413386) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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