Biotin Anti-TLR4 antibody [MTS510] (ab9105)
Key features and details
- Biotin Rat monoclonal [MTS510] to TLR4
- Suitable for: IHC-Fr, IP, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-TLR4 antibody [MTS510]
See all TLR4 primary antibodies -
Description
Biotin Rat monoclonal [MTS510] to TLR4 -
Host species
Rat -
Conjugation
Biotin -
Specificity
Only detects murine TLR-4 when complexed to MD-2. This antibody has been found to coprecipitate MD-2 (30kDa) with TLR4 (100kDa). -
Tested applications
Suitable for: IHC-Fr, IP, Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to TLR4. Mouse Ba/F3 cells expressing mouse TLR4
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General notes
Ab9105 is a TLR4 function blocking antibody that is useful for studies on the role of TLR4 as a receptor fpr LPS induced cytokine production by TLR4 bearing cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
0.2 µm filtered -
Primary antibody notes
Ab9105 is a TLR4 function blocking antibody that is useful for studies on the role of TLR4 as a receptor fpr LPS induced cytokine production by TLR4 bearing cells. -
Clonality
Monoclonal -
Clone number
MTS510 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Functional Studies
Use at an assay dependent concentration. |
Target
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Function
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
Tissue specificity
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
Involvement in disease
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Domain
The TIR domain mediates interaction with NOX4. -
Post-translational
modificationsN-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 21898 Mouse
- SwissProt: Q9QUK6 Mouse
- Unigene: 38049 Mouse
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Alternative names
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab9105 has been referenced in 2 publications.
- Chakraborty D et al. Alarmin S100A8 Activates Alveolar Epithelial Cells in the Context of Acute Lung Injury in a TLR4-Dependent Manner. Front Immunol 8:1493 (2017). PubMed: 29180999
- Daubeuf B et al. TLR4/MD-2 monoclonal antibody therapy affords protection in experimental models of septic shock. J Immunol 179:6107-14 (2007). Flow Cyt ; Mouse . PubMed: 17947685