PE Anti-TLR4 antibody [MTS510] (ab95563)
Key features and details
- PE Rat monoclonal [MTS510] to TLR4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
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Product name
PE Anti-TLR4 antibody [MTS510]
See all TLR4 primary antibodies -
Description
PE Rat monoclonal [MTS510] to TLR4 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length native protein (purified) corresponding to Mouse TLR4.
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Positive control
- 3-day thioglycolate-induced BALB/c macrophages; mouse peritoneal exudate cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MTS510 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.5µg for 105-8 cells.
Staining the cell sample in a final volume of 100 µL is recommended.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Notes |
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Flow Cyt
Use 0.5µg for 105-8 cells. Staining the cell sample in a final volume of 100 µL is recommended.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Function
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
Tissue specificity
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
Involvement in disease
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Domain
The TIR domain mediates interaction with NOX4. -
Post-translational
modificationsN-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 21898 Mouse
- SwissProt: Q9QUK6 Mouse
- Unigene: 38049 Mouse
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Alternative names
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
Images
Datasheets and documents
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Datasheet download
References (4)
ab95563 has been referenced in 4 publications.
- Chabot S et al. TLRs regulate the gatekeeping functions of the intestinal follicle-associated epithelium. J Immunol 176:4275-83 (2006). PubMed: 16547265
- Mortaz E et al. Acetylsalicylic acid-induced release of HSP70 from mast cells results in cell activation through TLR pathway. Exp Hematol 34:8-18 (2006). PubMed: 16413386
- Qi HY & Shelhamer JH Toll-like receptor 4 signaling regulates cytosolic phospholipase A2 activation and lipid generation in lipopolysaccharide-stimulated macrophages. J Biol Chem 280:38969-75 (2005). PubMed: 16176925
- Ortega-Cava CF et al. Strategic compartmentalization of Toll-like receptor 4 in the mouse gut. J Immunol 170:3977-85 (2003). PubMed: 12682225