Overview

  • Product name
    Anti-TLR5 antibody [19D759.2]
    See all TLR5 primary antibodies
  • Description
    Mouse monoclonal [19D759.2] to TLR5
  • Specificity
    This antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5.
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: SSKDFTWVQN ALLKHLDTQY SDQNRFNLCF EERDFVPGEN RIANIQDAIW NSRKIVCLVS RHFLRDGWCL EAFSYAQGRC LSDLNSALIM VVVGSLSQY QL conjugated to KLH, corresponding to amino acids 700-800 of Human TLR5.

  • Positive control
    • Ramos and Raw cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa.
Flow Cyt Use 2µg for 106 cells. Intracellular



ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Antigen retrieval with citrate buffer pH 6.
IHC-Fr Use a concentration of 10 µg/ml. (see Miller et al).
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • Function
    Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificity
    Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • Involvement in disease
    Systemic lupus erythematosus 1
  • Sequence similarities
    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Post-translational
    modifications
    Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10052 antibody
    • MGC126430 antibody
    • MGC126431 antibody
    • SLEB1 antibody
    • TIL 3 antibody
    • TIL3 antibody
    • TLR 5 antibody
    • Tlr5 antibody
    • TLR5_HUMAN antibody
    • Toll like receptor 5 antibody
    • Toll like receptor 5 precursor antibody
    • Toll-like receptor 5 antibody
    • Toll/interleukin 1 receptor like protein 3 antibody
    • Toll/interleukin-1 receptor-like protein 3 antibody
    see all

Anti-TLR5 antibody [19D759.2] images

  • All lanes : Anti-TLR5 antibody [19D759.2] (ab13876) at 2 µg/ml

    Lane 1 : Ramos cell lysate
    Lane 2 : Raw cell lysate


    Predicted band size : 103 kDa
  • Intracellular FACS analysis of TLR5 in 106 Ramos cells using (A) 2µg of isotype control and (B) 2µg of ab13876
  • ab13876 staining TLR5 in Human stomach tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 23°C; antigen retrieval was by heat mediation in citrate buffer (10mM, pH 6). Samples were incubated with primary antibody (10 µg/ml) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab13876 staining TLR5 in Human stomach tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (10 µg/ml in blocking buffer) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-TLR5 antibody [19D759.2] (ab13876)

This product has been referenced in:
  • Yamada K  et al. Upregulation of Toll-like receptor 5 expression in the conjunctival epithelium of various human ocular surface diseases. Br J Ophthalmol N/A:N/A (2014). Human . Read more (PubMed: 24820048) »
  • Dill-McFarland KA  et al. Hibernation alters the diversity and composition of mucosa-associated bacteria while enhancing antimicrobial defence in the gut of 13-lined ground squirrels. Mol Ecol 23:4658-69 (2014). Squirrel . Read more (PubMed: 25130694) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
macaque retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Mar 14 2017

Thank you for contacting us. An image of immunofluorescence can be found obn the website under the Abreviews section for ICC/IF. This product has not yet been used in immunofluorescence in a publication. The use of this product in ICC/IF in human or mo...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (NIH3T3 Mouse Fibroblasts)
Total protein in input
100 µg
Specification
NIH3T3 Mouse Fibroblasts
Immuno-precipitation step
Protein A/G
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Verified customer

Submitted Jan 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Intestine)
Loading amount
20 µg
Specification
Mouse Intestine
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Dec 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (NIH 3T3 Mouse Fibroblasts)
Specification
NIH 3T3 Mouse Fibroblasts
Preparation
Cell harvesting/tissue preparation method: Trypsin/centrifugation
Sample buffer: PBS 5%FCS
Fixation
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in PBS
Gating Strategy
Size gated NIH 3T3
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Submitted Sep 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (AGS human gastric carcinoma cells)
Total protein in input
200 µg
Specification
AGS human gastric carcinoma cells
Immuno-precipitation step
Protein A/G
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Submitted Jul 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (AGS Gastric Carcinoma cells)
Specification
AGS Gastric Carcinoma cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in TBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (AGS human gastric carcinoma cells)
Specification
AGS human gastric carcinoma cells
Preparation
Cell harvesting/tissue preparation method: Trypsin/centrifugation
Sample buffer: PBS 5%FCS
Fixation
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in PBS
Gating Strategy
Size gated AGS
Username

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Verified customer

Submitted Jul 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (AGS Gastric Carcinoma cells)
Specification
AGS Gastric Carcinoma cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in TBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jun 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
Loading amount
20 µg
Specification
AGS Gastric Carcinoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 17 2010

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