Our Abpromise guarantee covers the use of ab13876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa.|
|Flow Cyt||Use 2µg for 106 cells. Intracellular
ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Antigen retrieval with citrate buffer pH 6.|
|IHC-Fr||Use a concentration of 10 µg/ml. (see Miller et al).|
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|