Anti-TLR5 antibody [85B152.5] (FITC) (ab25929)


  • Product nameAnti-TLR5 antibody [85B152.5] (FITC)
    See all TLR5 primary antibodies
  • Description
    Mouse monoclonal [85B152.5] to TLR5 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH

  • Positive control
    • Ramos or ThP1 cells.



Our Abpromise guarantee covers the use of ab25929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 100 kDa.
Flow Cyt Use 2-5µg for 106 cells. (Intracellular)

ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • FunctionParticipates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • Tissue specificityHighly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • Involvement in diseaseSystemic lupus erythematosus 1
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • Post-translational
    Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10052 antibody
    • MGC126430 antibody
    • MGC126431 antibody
    • SLEB1 antibody
    • TIL 3 antibody
    • TIL3 antibody
    • TLR 5 antibody
    • Tlr5 antibody
    • TLR5_HUMAN antibody
    • Toll like receptor 5 antibody
    • Toll like receptor 5 precursor antibody
    • Toll-like receptor 5 antibody
    • Toll/interleukin 1 receptor like protein 3 antibody
    • Toll/interleukin-1 receptor-like protein 3 antibody
    see all

Anti-TLR5 antibody [85B152.5] (FITC) images

References for Anti-TLR5 antibody [85B152.5] (FITC) (ab25929)

This product has been referenced in:
  • Young BC  et al. Longitudinal expression of Toll-like receptors on dendritic cells in uncomplicated pregnancy and postpartum. Am J Obstet Gynecol N/A:N/A (2013). Human . Read more (PubMed: 24291497) »
  • Drewniak A  et al. Toll-like receptor-induced reactivity and strongly potentiated IL-8 production in granulocytes mobilized for transfusion purposes. Blood 115:4588-96 (2010). Flow Cyt ; Human . Read more (PubMed: 20354173) »

See all 5 Publications for this product

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Saponin will be OK to use however other e.g. Triton orDigitonin, can also be used.

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Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Haut, Kryoschnitte)
Specification Haut, Kryoschnitte
Fixative Acetone
Antigen retrieval step Other
Permeabilization Yes - 0,2% Triton X-100
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (primary activated T cells)
Specification primary activated T cells

Abcam user community

Verified customer

Submitted Aug 23 2006